摘要
Objective:To elucidate the expression of SPHK1 in gastric cancer tissues and to analyse its clinical value in gastric cancer using bioinformatics.Methods:The expression of SPHK1 in gastric cancer tissues was analysed using HPA and TCGA databases.The association between the expression of both SPHK1 and MKI67 in gastric cancer was analysed using GEPIA database.Based on Kaplan-Meier Plotter database to analyse the relationship between SPHK1 mRNA expression level and survival of gastric cancer patients.Gene enrichment pathway predicted the potential biological function of SPHK1 on gastric cancer.The TIMER database was used to predict the expression of SPHK1 in immune-infiltrating cells.The expression of SPHK1 in gastric cancer tissues was analysed by IHC and Western Blot.Results:Bioinformatics analysis showed that SPHK1 was highly expressed in gastric cancer tissues compared to paracancerous tissues(P<0.001).The analysis from the GEPIA database reveals a significant increase in the expression of SPHK1 and MKI67 in gastric cancer tissues(P<0.05).Furthermore,there is a positive correlation between the two proteins(P=0.00049).Kaplan-Meier Plotter survival analysis showed that high expression of SPHK1 predicted worse overall survival tine(logrankP=0.002)and post-progression generation time(logrankP=0.0019)in gastric cancer.Gene enrichment pathway results showed that SPHK1 may be involved in multiple gastric cancer progression pathways.TIMER database analysis showed that in macrophages,gastric cancer patients with low SPHK1 expression group had a better post-progression period than those with high SPHK1 expression group(P=0.002).IHC showed that the staining intensity and staining positive area of SPHK1 in gastric cancer tissues were higher than that of adjacent non-tumour tissues.And the staining intensity of TNMI and Ⅱ stages was weaker than that of Ⅲ and Ⅳ stages.Western blot analysis revealed higher levels of SPHK1 protein in gastric cancer tissue compared to paired adjacent non-cancerous tissue in 9 cases(P=0.0265).Conclusion:SPHK1 gene is highly expressed in gastric cancer tissues and is associated with poor prognosis and may reduce tumour immunosuppression,which may serve as a potential marker for evaluating the prognosis of gastric cancer patients.
基金
Anhui Province Higher Education Natural Science Research Project(No.KJ2021ZD0090)
Bengbu Medical College Graduate Research Innovation Project(No.Byycx22090)。
