Expression and significance of ADAM12 in bladder cancer

Objective:To investigate the expression and significance of ADAM12 in bladder cancer.Methods:Transcriptome data and clinical data of bladder cancer and normal samples from TCGA database were downloaded to analyze the expression level and prognosis of ADAM12 in bladder cancer and normal tissues,and analyze its clinical correlation.Bladder cancer wax block samples were collected to detect the expression level of ADAM12 protein in cancer and adjacent cancer by immunohisto-chemistry.In vitro experiments,the experiments were divided into siNC group and siADAM12 group by transfection interference sequence,and the effect of knockdown ADAM12 on the proliferation and migration of bladder cancer cells was evaluated by CCK8,Transwell and scratch experiments.Western Blot was used to detect the expression level of EMT-related protein in each group of bladder cancer cells.Bioinformatics was used to explore the potential mechanism of ADAM12 in influencing the progression of bladder cancer.Results:ADAM12 expression in bladder cancer tissue is higher than normal tissue(P<0.05).Compared with the low expression group,the prognosis of high-expression ADAM12 was worse(P=0.007),and it was related to tumor stage,depth of invasion and lymph node metastasis(P<0.05).The immunohistochemical results showed that ADAM12 was expressed higher in bladder cancer tissues than in neighboring tissues(P<0.05).The results of in vitro experiments showed that knocking down ADAM12 could inhibit the proliferation and migration of bladder cancer cells compared with the control group.Compared with the NC group,the Western Blot test results showed that the expression of E-cadherin was increased in the siADAM12 group,while the expression of N-cadherin and Vimentin was decreased.Overexpression leads to the opposite.Bioinformatics analysis showed that ADAM12 may be involved in multiple cancer-related signaling pathways and is associated with the infiltration of various immune cell(P<0.05).Conclusion:ADAM12 is highly expressed in bladder cancer tissues and is associated with tumor immune cell infiltration and promotes the migration of bladder cancer cells by regulating EMT,which may be a prognostic marker and biotherapeutic target for bladder cancer.