黑白中药通过调节MC1R和RTKs的表达对黑色素代谢的影响研究

Study on the Effect of Black and White Chinese Medicine on Melanin Metabolism by Regulating the Expression of MC1R and RTKs

目的:探讨首乌、女贞子等七味黑色中药和白及、白芷等七味白色中药对豚鼠皮肤组织黑色素合成中黑皮质素1受体(Melanocortin 1 receptor,MC1R)、受体酪氨酸激酶(Receptor tyrosine kinase,RTKs)表达的影响。方法:采用水提法获得中药提取物,用灌胃的方法以14 d为周期等量灌胃豚鼠获得背部皮肤组织,采用免疫组化及免疫荧光法检测实验和对照组MC1R、RTKs蛋白的表达,用ImageJ软件统计分析结果,比较不同中药环境对豚鼠皮肤组织的黑色素合成的影响,分析MC1R、RTKs蛋白与黑素细胞代谢三者的变化关系。结果:对MC1R蛋白有抑制作用的白药有白芷、白茯苓、白蔹、白及、白僵蚕、白术,其中白芷、白僵蚕、白术的作用最为明显,对MC1R有明显抑制作用的黑药有乌梅、黑芝麻、丹参、鸡血藤,其中丹参的抑制作用最为明显。该结果显示上述10味中药对黑色素合成有抑制作用,其中白芷、白僵蚕、白术、丹参的抑制作用最为明显。其中对于RTKs蛋白有抑制作用的白药有白芷、白茯苓、白蔹、白及、白僵蚕,其中白茯苓、白蔹、白及的作用最为明显,具有明显作用的黑药有黑芝麻、丹参,其中丹参的作用最为明显。结论:药材颜色与药效之间有一定关系;黑白中药都对MC1R及RTKs蛋白有不同程度的抑制作用,色象中药可能通过调节α-黑素细胞剌激素/黑皮素1受体(Alphamelanocyte stimulating hormone/Melanocortin 1 receptor,α-MSH/M1CR)、干细胞生长因子/受体酪氨酸激酶(Stem cell growth factor/Receptor tyrosine kinase,SCF/RTKs)信号通路来影响黑素细胞代谢。

Objective To investigate the effects of seven black Chinese herbs, such as Polygonum multiflorum Thunb, and Ligustrum lucidum, and seven white Chinese herbs, such as Bletilla striata and Angelica dahurica, on the expression of melanocortin 1 receptor(MC1R) and receptor tyrosine kinase(RTKs) in melanin synthesis of guinea pig skin. Methods The extract of traditional Chinese medicine was obtained by water extraction, and the skin tissue of the back of guinea pigs was obtained by gavage of the same amount in a 14 day cycle. The expression of MC1R protein and RTKs protein in the experimental and control groups were detected by immunohistochemistry and fluorescence methods. The results were statistically analyzed by Image J software. The effects of different traditional Chinese medicine environments on melanin synthesis in the skin tissue of guinea pigs were compared, and MC1R The relationship between RTKs protein and melanocyte metabolism. Results The white herbs with inhibitory effect on MC1R protein are Angelica dahurica, Poria cocos, Ampelopsis japonica, Atractylodes macrocephala, atractylodes macrocephala, among which the effects of Angelica dahurica, Atractylodes macrocephala, Atractylodes macrocephala are the most obvious, and the black herbs with significant inhibitory effect on MC1R are Ophiopogon flexuosus, Nigella sativa, Dangshen, Chickweed, among which the inhibitory effect of Dangshen is the most obvious. This result showed that the above 10 herbs had inhibitory effects on melanin synthesis, among which the inhibitory effects of Angelica dahurica, Cortex alba, Atractylodes macrocephala, and Salvia miltiorrhiza were the most obvious. Among them, the white herbs with inhibitory effect on RTKs protein are Angelica dahurica, Poria cocos, Ampelopsis pilosulae,Bupleurum officinale and Bupleurum officinale, among which Poria cocos, Ampelopsis pilosulae and Bupleurum officinale have the most obvious effect, and the black herbs with obvious effect are Nigella sativa and Salvia miltiorrhiza, among which Salvia miltiorrhiza has the most obvious effect. Conclusion There is a certain relationship between the color of medicinal materials and their efficacy. Both black and white drugs inhibit the action of MC1R and RTKs protein to varying degrees, and color image Chinese medicine may regulate α-MSH/M1CR and SCF/RTKs signal pathways affect melanocyte metabolism.