高产γ-氨基丁酸植物乳杆菌的筛选、鉴定及益生特性

Screening,Identification,and Probiotic Characterization of a Lactobacillus plantarum Strain with a High Yield ofγ‐Aminobutyric Acid

从东北酸菜和韩国泡菜中筛选高产γ-氨基丁酸的植物乳杆菌菌株,对其测序鉴定乳酸菌种类,并深入研究该菌株的益生特性。结果表明:通过16S rDNA测序鉴定该菌株为植物乳杆菌,命名为Lactobacillus plantarum Lp3。该菌株有良好的生长和产酸能力,接种2~12 h生长较快进入对数期,接种0~6 h快速产酸。益生特性表明:L. plantarum Lp3菌株对大肠杆菌、沙门氏菌、金黄色葡萄球菌均有较好的抑制效果,对沙门氏菌抑菌圈直径达32.27 mm。当胆盐浓度为0.5%和pH值为2.0时,L. plantarum Lp3菌株活菌数分别为7.12 lg(CFU/mL)和7.29 lg(CFU/mL),说明其具有良好的耐胆盐及耐酸能力。同时,L. plantarum Lp3菌株在胃肠道中表现出很好的生存能力,对常用的红霉素、氯霉素和四环素等抗生素具有一定的敏感性。

A Lactobacillus plantarum strain with a high yield of gamma-aminobutyric acid(GABA)was isolated and screened from pickled Chinese cabbage and Korean kimchi and identified by 16S rDNA sequencing.Furthermore,the probiotic properties of the strain were characterized.The results showed that the strain was identified as L.plantarum and named as L.plantarum Lp3.The strain grew rapidly into the logarithmic phase 2-12 h after inoculation and rapidly produced acids during 0-6 h after inoculation,which indicated good growth and acid-producing ability.Furthermore,L.plantarum Lp3 had strong inhibitory effects on Escherichia coli,Salmonella,and Staphylococcus aureus,and the diameter of the inhibition zone against Salmonella reached 32.27 mm.The viable counts of L.plantarum Lp3 were 7.12 lg(CFU/mL)and 7.29 lg(CFU/mL)at the bile salt concentration of 0.5%and pH2.0,respectively,indicating that the strain had strong bile salt and acid tolerance.Meanwhile,L.plantarum Lp3 showed high viability in the gastrointestinal tract and exhibited sensitivity to antibiotics such as erythromycin,chloramphenicol,and tetracycline.