BALB/cSpf小鼠和野生型BALB/c小鼠生物学特性比较

Comparison of Biological Characteristics of BALB/cSpf Mice and wild-type BALB/c mice

目的比较分析BALB/cSpf小鼠与野生型BALB/c(N-BALB/c,N:normal)小鼠生物学特性指标差异,为其实验应用提供基础数据。方法3~12周,每周对雌雄BALB/cSpf小鼠和N-BALB/c小鼠生长曲线、采食量、饮水量进行统计分析;取BALB/cSpf小鼠雌性和N-BALB/c小鼠雌性各30只,二雌一雄长期同居交配方式繁殖饲养,记录产仔数,产仔性别;采集血液进行17项血液生化指标和22项血液生理指标测定。结果BALB/cSpf小鼠和N-BALB/c小鼠采食量和饮水量差异不显著(P>0.05),BALB/cSpf小鼠体质量显著高于N-BALB/c小鼠(雌性从第5周开始,雄性从第3周开始)。1~3胎产仔数及产仔性别差异不显著。雌性BALB/cSpf小鼠生理指标WBC、Neu、Lym、RBC、HGB、HCT、MPV、PDW显著高于N-BALB/c小鼠(P<0.01)。雄性BALB/cSpf小鼠生理指标WBC、Neu、Lym、Mon、HCT、MCHC显著低于N-BALB/c小鼠(P<0.01)。雌性BALB/cSpf小鼠生化指标UREA显著高于N-BALB/c小鼠(P<0.05);AST、CK、LDH显著低于N-BALB/c小鼠(P<0.01)。BALB/cSpf小鼠生化指标TG、TC、LIP、UREA、MgⅡ显著高于N-BALB/c小鼠(P<0.01);ALT、AST、Glu-G、Ca显著低于N-BALB/c小鼠(P<0.01)。结论BALB/cSpf小鼠和N-BALB/c小鼠生物学特性有一定差异。

Objective To compare and analyze the biological characteristics of BALB/cSpf mice and wild-type BALB/c(N-BALB/c,N:normal)mice,and to provide basic data for its experimental application.Method From 3 to 12 weeks,the growth curves,feed intake and water intake of male and female BALB/cSpf mice and N-BALB/c mice were statistically analyzed weekly.Thirty female BALB/cSpf mice and thirty female N-BALB/c mice were collected,two females and one male cohabitated and bred for a long time.The litter size and litter sex were recorded.Blood was collected and 17 blood biochemical indexes and 22 blood physiological indexes were measured.Result there was no significant difference in feed intake and drinking water between BALB/cSpf mice and N-BALB/c mice.The body weight of BALB/cSpf mice was significantly higher than that of N-BALB/c mice(from the 5th week in females and from the 3rd week in males).There was no significant difference in litter size and sex in the first three births.The physiological indexes of WBC,Neu,Lym,RBC,HGB,HCT,MPV and PDW in female BALB/cSpf mice were significantly higher than those in N-BALB/c mice.The WBC of male BALB/cSpf mice was significantly higher than that of N-BALB/c mice,while the levels of Neu,Lym,Mon,HCT and MCHC were significantly lower than those of N-BALB/c mice.The UREA of female BALB/cSpf mice was significantly higher than that of N-BALB/c mice,while the levels of AST,CK and LDH were significantly lower than those of N-BALB/c mice.The biochemical indexes TG,TC,LIP,UREA and MgⅡof BALB/cSpf mice were significantly higher than those of N-BALB/c mice,while ALT,AST,Glu-G and Ca were significantly lower than those of N-BALB/c mice.Conclusion there are some differences in biological characteristics between BALB/cSpf mice and N-BALB/c mice.