子宫内膜异位组织中miR-210、miR-486水平与患者手术后妊娠关系

Correlation between the microrna ribonucleic acid 210 and microribonucleic acid 486 levels in the ectopic endometrial tissue of patients with endometriosis and their pregnancy after surgery

目的:探究子宫内膜异位症(EMS)患者子宫内膜组织中微小核糖核酸-210(miR-210)、微小核糖核酸-486(miR-486)水平及与术后妊娠关系。方法:选取2020年2月-2022年2月本院收治的100例经腹腔镜手术治疗的EMS患者临床资料,根据术后妊娠情况分为妊娠组(n=62例)和非妊娠组(n=38例)。采用实时定量聚合酶链反应(qRT-PCR)检测子宫内膜组织中miR-210、miR-486水平。采用受试者工作特征(ROC)曲线分析miR-210、miR-486水平对EMS患者术后妊娠的预测价值;采用Spearman相关性分析miR-210、miR-486水平与EMS患者生育指数(EFI)评分关系。结果:非妊娠组子宫内膜组织中miR-210(1.24±0.18)、miR-486(1.21±0.17)水平均高于妊娠组(1.01±0.11、1.02±0.12),且临床分期为Ⅳ期的子宫内膜组织miR-210、miR-486水平高于Ⅰ期、Ⅱ期者(均P<0.05)。miR-210、miR-486水平联合检测预测EMS患者术后妊娠的曲线下面积(0.955)升高(P<0.05)。Spearman法分析显示,EMS患者子宫内膜组织miR-210、miR-486水平与EFI评分均为负相关关系(r=-0.541、-0.429,均P<0.05)。结论:EMS患者子宫内膜组织miR-210、miR-486高表达,且与术后妊娠有关。

Objective:To investigate the correlation between the levels of the microribonucleic acid 210(miR-210)and miR-486 in the ectopic endometrial tissue of patients with endometriosis(EMS)and their pregnancy after surgery.Methods:The clinical data of 100 patients with EMS who had undergone laparoscopic surgery from February 2020 to February 2022 were selected in this study.According to the pregnancy status,these patients were divided into group A(62 cases with pregnancy)and group B(38 cases without pregnancy).Real-time quantitative polymerase chain reaction(qRT-PCR)was applied to detect the miR-210 and miR-486 levels in the ectopic endometrial tissue.Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of the miR-210 and miR-486 levels in the ectopic endometrial tissue of the patients with EMS for their pregnancy outcomes.Spearman correlation was applied to analyze the correlation between the levels of miR-210 and miR-486 levels in the ectopic endometrial tissue of the patients with EMS and their endometriosis fertility index(EFI)score.Results:The levels of miR-210(1.24±0.18)and miR-486(1.21±0.17)in the ectopic endometrial tissue of the patients in group B were significantly higher than those(1.01±0.11 and 1.02±0.12)of the patients in group A.The levels of miR-210 and miR-486 in the ectopic endometrial tissues of the patients with stage Ⅳ of EMS were significantly higher than those of the patients with stage Ⅰ and Ⅱ of EMS(all P<0.05).The area under the curve(0.955)of the combined detection of miR-210 and miR-486 levels of the patients with EMS after surgery for predicting their postoperative pregnancy was 0.955,which was significantly higher than that of the miR-210 level or the miR-486 level alone(P<0.05).Spearman analysis showed that the miR-210 level and the miR-486level in the ectopic endometrial tissue of the patients with EMS were negatively correlated with their EFI score(r=-0.541,-0.429,all P<0.05).Conclusion:The expressions of miR-210and miR-486in the ectopic endometrial tissue of the patients with EMS are high,and which are related to the pregnancy of the patients after surgery.