胶体金免疫层析法快速检测中药材中除草剂扑草净残留

Colloidal gold immunochromatographic assay for rapid detection of herbicide prometryn in traditional Chinese medicine

目的:基于自制的扑草净抗原和单克隆抗体,建立了一种适用于快速检测中药材、饮片中三嗪类除草剂扑草净残留的免疫层析方法。方法:确定了抗体偶联量为7μg,稀释液配制条件为含1%OVA,0.1%Tween-80的0.1 mol·L^(-1) PBS优化条件,并采用T线划线浓度为0.05 mg·mL^(-1),C线划线浓度为0.5 mg·mL^(-1)的湿法试纸条,提升胶体金免疫层析试纸条的灵敏度,降低不同用药部位中药的基质效应,并进行大批量中药材检测。结果:试纸条检测灵敏度可达到1 ng·mL^(-1),实际样品检出限可达到0.1 mg·kg^(-1),所建立的方法适用于陈皮、蒲公英、三七、金银花等中药材中40种不同部位扑草净残留检测。对10批西洋参、5批山药、12批三七、5批黄芪、3批沙参、4批牛膝、14批菊花、9批生姜、6批菟丝子进行了实际样品快速检测,均未检出扑草净残留。结论:本方法检测方法快速准确,前处理简便,操作简单,推广性强,可作为现场快速筛查扑草净残留的有效手段,以保证中药材的质量和安全性。同时,该研究为开发多种中药材的农残快速检测具有一定的参考意义。

Objective:To establish an immunochromatographic method suitable for the rapid detection of residues of triazine herbicide prometryn in Chinese medicine, based on self-made prometryn antigen and monoclonal antibody. Methods: An antibody conjugate amount of 7 μg and dilution configuration of 0.1 mol·L^(-1) PBS with 1%OVA, 0.1% Tween-80 were determined, and dry test strips with a T-line scribing concentration of 0.05 mg·mL^(-1) and a C-line scribing concentration of 0.5 mg·mL^(-1) were used. The suitable method was selected to improve the sensitivity of colloidal gold immunochromatography test strip, reduce the matrix effect of traditional Chinese medicine in different parts, and detect a large number of traditional Chinese medicine. Results: The detection sensitivity of the test strip could reach 1 ng·mL^(-1), and the detection limit of the actual sample could reach 0.1 mg·kg^(-1). The established method was suitable for the detection of prometryn residues in 40 kinds of Chinese medicinal materials such as Citri Reticulatae Pericarpium, Taraxaci Herba, Notoginseng Radix et Rhizoma andLonicerae Japonicae Flos. Conclusion: This method has the advantages of rapid and accurately detection, simple pretreatment, simple operation and strong generalization, and can be used as an effective method for rapid screening of prometryn residues in the field, so as to ensure the quality and safety of traditional Chinese medicine. At the same time, this study has a certain reference significance for the development of rapid detection of agricultural residues in many kinds of traditional Chinese medicine.