Macrophage-derived exosomes mediate mir-222 targeting Caspase-10 to promote glioma proliferation

Objective:To investigate whether macrophage-derived exosomal mir-222 regulates Caspase-10 and whether macrophage-derived exosomes promote glioma proliferation by targeting Caspase-10 through mir-222.Methods:Macrophages were transfected with mir-222 mimics,and then macrophage-derived exosomes carrying mir-222 were transfected into glioma cells.Caspase-10 overexpression vector was constructed on this basis.RT-PCR was used to detect mir-222 expression in macrophages and glioma cells.CCK-8 assay was used to detect the proliferative activity of glioma cells.Dual-luciferase reporter assay was used to verify the targeting binding of mir-222 with Caspase-10 gene.Western blot was used to detect Caspase-10 expression.Results:Transmission electron microscopy showed that macrophagederived exosomes had spherical structures with a diameter of about 100 nm;mir-222 mimic significantly promoted the expression of mir-222 in macrophages;macrophage-derived exosomal mir-222 increased mir-222 expression and promoted proliferation in glioma cells;mir-222 could target bind with Caspase-10;mir-222 mimic significantly inhibited Caspase-10 expression.Conclusion:Macrohpage-derived exosomal mir-222 plays an important role in promoting glioma proliferation by downregulating Caspase-10 expression through targeted binding.