玉米PI4K基因家族的鉴定及生物信息学分析

Identification and Bioinformatics Analysis of PI4K Gene Family in Maize

磷脂酰肌醇4-激酶(Phosphatidylinositol 4-kinase,PI4K)作为磷脂酰肌醇信号通路代谢中的关键酶,在植物的生长过程中发挥着重要作用。通过生物信息学的方法从玉米数据库中鉴定出13个ZmPI4K家族成员,通过系统进化、motif、基因结构、组织表达模式、顺式作用元件、诱导表达、互作蛋白对ZmPI4Ks成员进行分析。结果表明,玉米ZmPI4K家族分为3个亚家族γ、β、α;同属于一个亚家族成员的motif和基因结构相似。ZmPI4Ks各成员在不同组织均有表达,顺式作用元件和诱导表达结果显示,ZmPI4Ks可能参与植物的生长发育且响应环境或激素胁迫。互作蛋白预测结果暗示,ZmPI4Ks成员可能通过与脂质合成及转运相关蛋白互作来响应非生物胁迫。

Phosphatidylinositol 4-kinase(PI4K),a key enzyme in the metabolism of phosphatidylinositol signal⁃ing pathway,plays an important role in the growth of plants.Thirteen ZmPI4K family members were identified from maize databases by bioinformatics,and the members of ZmPI4Ks were analyzed by phylogenetic,motif,gene struc⁃ture,tissue expression patterns,cis-acting elements,induced expression,and reciprocal proteins.The results showed that the maize ZmPI4K family was divided into three subfamilies:γ,β,andα.The motifs and gene struc⁃tures of the members belonging to the same subfamily were similar.ZmPI4Ks members were expressed in different tissues.The results of cis-acting elements and induced expression suggest that ZmPI4Ks may be involved in plant growth and development in response to environmental or hormonal stress;the results of interacting protein predic⁃tion imply that ZmPI4Ks members may respond to abiotic stress by interacting with proteins involved in lipid synthe⁃sis and transporting.This study aims to provide a theoretical basis for exploring the PI4K family of maize and other species,and provide genetic resources for molecular breeding.