红蓝光诱导下红地球葡萄VvBES1-1基因表达模式分析

Gene Expression Patterns of VvBES1-1 in Red Globe Grapes Induced by Red and Blue Lights

BRI1-EMS-Suppressor 1(BES1)是油菜素内酯信号转导重要的转录因子,已被证实在调控植物光形态建成及花芽发育方面发挥重要作用。为探究VvBES1是否响应红蓝光调控红地球葡萄花芽分化及在该调控模式下VvBES1的表达规律,本研究以红地球葡萄花芽为试验材料,温室自然光作为对照(CK),红蓝4∶1(R4B1)为光照处理,从前期转录组数据筛选获得VvBES1-1(Vitvi10g00636),同源克隆获得该基因编码序列(CDS),全长1026 bp,编码341个氨基酸;生物信息学分析结果显示,VvBES1-1蛋白含有1个BES1家族高度保守的BES1-N结合结构域,与河岸葡萄属于同一亚族。VvBES1-1表达具有组织特异性,VvBES1-1时空表达模式显示,VvBES1-1在各时期均有一定表达,但是在R4B1处理期间表达量显著低于自然光,9月15日(花序二级轴发育期)表达量达到最高,由此推测VvBES1-1在红地球葡萄花序二级轴发育期发挥重要作用。VvBES1-1烟草异源转化试验结果显示,转基因植株表现出花期延迟、节间缩短等表型特征,外源BR处理下VvBES1-1基因表达量均明显上调表达,VvBES1-1在光介导控花芽分化的过程中表达模式呈先升后降趋势且同红地球葡萄中的一致。本研究结果为VvBES1-1参与红蓝光介导BR信号调控红地球葡萄花芽分化提供了理论依据。

BRI1-EMS-Suppressor 1(BES1),a crucial transcription factor involved in oleuropein lactone signaling,has been demonstrated to play a significant involvement in the regulation of plant photomorphogenesis and bud development.In order to investigate whether VBESI regulates flower bud differentiation through responding to red and blue light in Red Globe grapes,as well as the expression pattern of VuBES1 under this mode of regulation.In this study,red globe grape buds were utilized as test materials,while a 4:1 ratio of red and blue light(R4B1)was used as the light treatment and greenhouse natural light served as the control(CK).Through screening from transcriptome data generated before,VoBES1-1(Vitvi1Og00636)was selected,and its coding sequence(CDS)was obtained via homologous cloning with a total length of 1026 bp,encoding 341 amino acids.Bioinformatics analysis revealed that VvBES1-1 protein contained one highly conserved BES1-N binding structural domain of the BES1 family,belonging to the same subfamily as riverbank grapes.The expression pattern of VvBES1-1 showed tissue-specificity and continuous expression throughout all periods;However,its expression during R4B1 treatment period was significantly lower than that under natural light conditions.On September 15th,VuBES1-1 reached its highest expression level,coinciding with the developmental phase of secondary axis in red globe grapevine inflorescence.Therefore,it is hypothesized that VuBES1-1 plays a crucial role during this developmental period.Tobacco heterologous transformation assay demonstrated delayed flowering and shortened internodes in transgenic plants expressing VuBES1-1 gene,indicating altered phenotypic traits.Upon treatment with exogenous BR,the expression of the VvBESI-l gene was upregulated.The expression of VvBESI-1 was increased first and then decreased,which was consistent with the light-mediated regulation of floral differentiation in Red Globe grapevine.In conclusion,this study provides a theoretical framework elucidating the role of VoBESI-l in red and blue light-mediated BR signaling for controlling flower bud differentiation in the Red Globe grape.