转录组和蛋白组联合揭示甜瓜响应涝害胁迫的分子机制

Joint Analysis of Transcriptome and Proteome Revealed the Molecular Mechanism of Melon(Cucumis melo L.)in Response to Waterlogging Stress

为了探析甜瓜(Cucumis melo L.)响应涝害胁迫的分子机制,采用转录组和蛋白组学方法研究了甜瓜耐涝型材料L45和涝敏感型材料L39在正常培养和涝害胁迫处理条件下的差异表达基因和蛋白。结果表明,L39-W vs L39-C的差异表达基因数为3532个,差异表达蛋白数为105个;L45-W vs L45-C的差异表达基因数为1842个,差异表达蛋白数为38个。转录组与蛋白组联合分析发现,L39-W vs L39-C中38个基因在mRNA和蛋白质水平上联合上调表达,12个基因联合下调表达;L45-W vs L45-C中7个基因联合上调表达,3个基因联合下调表达。KEGG显著性富集分析结果发现,L39-W vs L39-C中联合上调表达的基因显著富集在类黄酮生物合成、苯丙烷生物合成、苯丙氨酸代谢、缬氨酸、亮氨酸和异亮氨酸生物合成、精氨酸和脯氨酸代谢等;而L45-W vs L45-C中联合上调表达的基因显著富集在α-亚麻酸代谢、类黄酮生物合成、苯丙烷生物合成、亚油酸代谢等。本研究联合转录组与蛋白组数据进行了差异表达基因与蛋白分析,为进一步挖掘甜瓜响应涝害胁迫的关键基因及通路奠定了一定基础。

To investigate the molecular mechanism of melon(Cucumis melo L.)in response to waterlogging stress,we conducted a comparative analysis of the transcriptome and proteome of the waterlogging-sensitive accession L39 and the waterlogging-tolerant accession L45 under normal water supply and waterlogging stress conditions.In the leaves of waterlogging-stressed L39,a total of 3532 genes showed differential expression compared to the control,while in L45,1842 genes showed differential expression.Additionally,we identified 105 and 38 differentially expressed proteins in the comparison groups L39-W vs L39-C and L45-W vs L45-C,respectively.Comparing the transcriptome and proteome datasets,we observed that 38 and 12 genes exhibited jointly up-regulated and down-regulated expression profiles at the mRNA and protein levels in the L39-W vs L39-C comparison,whereas 7 genes showed co-up-regulated expression and three genes displayed co-downregulated expression in the L45-W vs L45-C comparison.The KEGG pathway analysis revealed that the co-upregulated genes in the L39-W vs L39-C comparison were mainly involved in‘flavonoid biosynthesis’,‘phenylpropanoid biosynthesis’,‘phenylalanine metabolism’,‘valine,leucine and isoleucine biosynthesis’,and‘arginine and proline metabolism’.In contrast,the co-up-regulated pathways in the L45-W vs L45-C comparison were predominantly related to‘α-linolenic acid metabolism’,‘flavonoid biosynthesis’,‘phenylpropanoid biosynthesis’,and‘linoleic acid metabolism’.By combining transcriptome and proteome data,this study provides a foundation for further exploration of key genes and pathways involved in the response of melon to waterlogging stress.