球茎甘蓝qRT-PCR内参基因的筛选及稳定性验证

Screening and stability verification of kohlrabi reference genes

【目的】通过对已知内参基因进行筛选,确定球茎甘蓝最合适的内参基因,确保实时荧光定量PCR(qRT-PCR)的准确表达。【方法】本试验选择了11种参考基因,利用GeNorm、Normalfinder和Bestkeep软件对紫色球茎甘蓝和绿色球茎甘蓝不同部位的内部参考基因进行了分析。【结果】3个分析结果显示,内参基因Tip41在球茎甘蓝不同组织部位表达最稳定。【结论】Tip41是作为内参基因的最佳选择,为球茎甘蓝后续的分子生物学相关研究提供基础。

【Objective】By screening known reference genes,the most appropriate reference genes in cabbage were identified to ensure the accurate expression of quantitative real-time PCR(QRT-PCR).【Method】Eleven reference genes were selected in this experiment,and the internal reference genes of different parts of purple and green cabbage were analyzed by GeNorm,Normalfinder and Bestkeep software.【Result】Three analysis results showed that the internal reference gene Tip41 was expressed most stably in different tissue parts of kohlrabi.【Conclusion】Tip41 was the best choice as an internal reference gene,provide a basis for the subsequent molecular biology related studies of kohlrabi.