miR-263干扰对麦长管蚜生命表参数的影响

Effects of miR-263 interference on the life table parameters of the English grain aphid, Sitobion avenae (Hemiptera: Aphididae)

【目的】麦长管蚜Sitobion avenae是小麦生产的首要害虫。本研究旨在明确miR-263干扰对麦长管蚜室内种群的影响,为小麦蚜虫的绿色防控提供技术支持。【方法】以纳米材料为载体,利用400 nmol/L miR-263 agomir干扰麦长管蚜1龄若蚜,采用两性生命表评价miR-263干扰对其生长发育、繁殖和种群增长的影响,以清水阴性对照(water negative control, NCW)和纳米材料阴性对照(nanomaterial negative control, NCS)作为对照组。【结果】miR-263干扰后,与NCW和NCS相比,麦长管蚜1和2龄若蚜历期显著延长,3龄若蚜历期没有显著差异,4龄若蚜历期要显著短于NCW组,整个若蚜历期显著延长;全生育期(25.42 d)显著长于NCS的(23.73 d),与NCW (23.84 d)没有显著差异;成蚜寿命和繁殖历期有所延长,单雌产蚜量有所增加,但与NCW和NCS的比没有显著差异。miR-263干扰后麦长管蚜成蚜前存活率为43.33%,显著低于NCW和NCS的(分别为91.34%和91.32%)。种群生命表数据显示,miR-263干扰后内禀增长率(r)、周限增长率(λ)、净增殖率(R_0)均显著低于NCW和NCS的,平均世代周期(T)比NCW和NCS显著延长;种群预测结果显示,在miR-263干扰60 d时麦长管蚜种群约为8万头,显著低于NCS的454万头和NCW的394万头。【结论】miR-263干扰主要影响低龄若蚜的历期和存活率,并对麦长管蚜的种群增长具有抑制作用,研究结果为麦长管蚜RNAi生物农药的研发提供了候选基因。

【Aim】The English grain aphid,Sitobion avenae is a destructive pest in wheat production.This study aims to clarify the effects of miR-263 interference on the laboratory population of S.avenae,so as to provide a theoretical basis for the green prevention and control of wheat aphids.【Methods】The 1st instar nymphs of S.avenae were interfered with 400 nmol/L miR-263 agomir using nanomaterials as carriers,and the effects of miR-263 interference on the growth and development,reproduction,and population growth of S.avenae were evaluated using the two-sex life table.The water negative control(NCW)and nanomaterial negative control(NCS)were employed as the control groups.【Results】After miR-263 interference,the duration of both the 1st and 2nd instar nymphs of S.avenae was significantly longer than that of the NCW and NCS,that of the 3rd instar nymphs showed no significant difference from that of the NCW and NCS,and that of the 4th instar nymphs was significantly shorter than that of NCW.The entire nymphal duration was significantly extended after miR-263 interference.After miR-263 interference,the total developmental duration(25.42 d)was significantly longer than that of the NCS(23.73 d),but had no significant difference from that of the NCW(23.84 d).After miR-263 interference,the adult longevity and reproduction duration were slightly extended,and the number of offspring produced per female was increased,but showed no significant difference from those of the NCW and NCS.The pre-adult survival rate of S.avenae after miR-263 interference was significantly decreased to 43.33%as compared with those of the NCW and NCS(91.34%and 91.32%,respectively).The life table data showed that the intrinsic rate of increase(r),finite rate of increase(λ),and net reproductive rate(R 0)after miR-263 interference were significantly lower than those of the NCW and NCS.The mean generation time(T)after mir-263 interference was significantly prolonged as compared to that of the NCW and NCS.The population prediction results showed that the population size of S.avenae was approximately 80000 at 60 d after miR-263 interference,significantly lower than the population sizes of 4.54 million of the NCS and 3.94 million of the NCW.【Conclusion】The miR-263 interference mainly affected the duration and survival rate of low instar nymphs,and had an inhibitory effect on the population growth of S.avenae.The results provide candidate genes for the development of RNAi-based biopesticides against S.avenae.