不同微生物制剂对大口黑鲈生长、形体指标及肠道组织结构的影响

Effects of Different Microbial Preparations on the Growth,Physical Indices andIntestinal Tissue Structure of Micropterus salmoides

为探究不同微生物制剂对大口黑鲈生长、形体指标及肠道组织结构的影响,在商业配合饲料中添加不同浓度的丁酸梭菌[0(A组,CK)、0.25%(B组)、0.5%(C组)、1%(D组)丁酸梭菌(5×10^(8) cfu·g^(-1))]、0.2%酵母产品(E组)及两者的混合物(0.25%丁酸梭菌+0.2%酵母产品,F组)进行两阶段共17周的养殖试验。结果表明:第一阶段(14周)试验组中,除添加0.2%酵母产品(E组)外,其余均较对照显著提升了大口黑鲈的生长性能,其中添加0.5%丁酸梭菌效果最优;第二阶段(17周)试验组间(A、C和F组),0.5%丁酸梭菌(C组)和0.25%丁酸梭菌+0.2%酵母产品(F组)的末均重及末体长较对照A组均显著增加;肥满度、脏体比指数、肠绒毛长度及肌层厚度三组间无显著差异,但与对照(A组)相比,添加0.5%丁酸梭菌(C组)的各指标有增高趋势,添加0.25%丁酸梭菌+0.2%酵母产品(F组)的各指标有降低趋势。研究表明,在本试验条件下,商业配合饲料中添加0.5%丁酸梭菌对大口黑鲈生长及肠道健康改善的效果最佳。

In order to explore the effects of different microbial preparations on the growth,body index and intestinal tissue structure of Micropterus salmoides,different concentrations of Clostridium butyricum[0(group A,CK),0.25%(group B),0.5%(group C),1%(group D)Clostridium butyricum(5×10^(8)cfu·g^(-1))],0.2%yeast products(group E)and their mixture(0.25%Clostridium butyricum+0.2%yeast products,group F)were added to the commercial compound feed for a two-stage breeding experiment for 17 weeks.The results showed that:in the first stage(14 weeks),except for adding 0.2%yeast products(group E),the growth performance of Micropterus salmoides was significantly improved compared with the control group,and the effect of adding 0.5%Clostridium butyricum was the best.In the second stage(17 weeks),the final mean weight and final body length of 0.5%Clostridium butyricum(group C)and 0.25%Clostridium butyricum+0.2%yeast products(group F)were significantly higher than those of the control group A.There were no significant differences in the fullness,visceral-body ratio index,length of intestinal villus and muscle layer thickness among the three groups.However,compared with the control group(group A),the indexes of adding 0.5%Clostridium butyricum(group C)had an increasing tendency,and the indexes of adding 0.25%Clostridium butyricum+0.2%yeast products(group F)had a decreasing tendency.The results showed that under the experimental conditions,the addition of 0.5%Clostridium butyricum to the commercial compound feed had the best effect on the growth and improvement of intestinal health of Micropterus salmoides.