口腔鳞状细胞癌组织中IDO1、KYNU表达情况与临床病理特征及预后的关系

Relationship between the expression of IDO1 and KYNU in oral squamous cell carcinoma tissues and clinicopathologic features and prognosis

目的探讨口腔鳞状细胞癌(OSCC)组织中吲哚胺2,3-双加氧酶1(IDO1)、犬尿氨酸酶(KYNU)表达情况与病理特征、预后的关系。方法将2018年1月至2020年6月该院收治的98例OSCC患者纳入研究。收集患者OSCC组织标本及对应的癌旁组织标本。采用免疫组化法检测组织中IDO1、KYNU的表达情况,分析IDO1、KYNU表达情况与OSCC患者临床病理特征的关系。采用多因素Cox回归分析OSCC患者预后的影响因素。结果OSCC组织IDO1、KYNU阳性表达率高于癌旁组织(P<0.05)。OSCC分化程度低分化、浸润深度(DOI)>5 mm、淋巴结转移、临床分期Ⅲ~Ⅳ期的患者IDO1、KYNU阳性表达率分别高于OSCC分化程度中高分化、DOI≤5 mm、淋巴结无转移、临床分期Ⅰ~Ⅱ期的患者(P<0.05)。OSCC分化程度中高分化、DOI≤5 mm、淋巴结无转移、临床分期Ⅰ~Ⅱ期、IDO1阴性、KYNU阴性患者的3年总生存率分别高于OSCC分化程度低分化、DOI>5 mm、淋巴结转移、临床分期为Ⅲ~Ⅳ期、IDO1阳性、KYNU阳性患者(P<0.05)。多因素Cox回归分析显示,DOI>5 mm(HR=3.225,95%CI:1.496~6.954),IDO1阳性(HR=3.714,95%CI:1.941~7.105),KYNU阳性(HR=4.150,95%CI:1.887~9.124)是OSCC患者预后的影响因素(P<0.05)。结论IDO1、KYNU在OSCC患者癌组织中呈高表达,与OSCC的DOI、临床分期、分化程度等特征密切相关,有望作为辅助评估患者预后的标志物。

Objective To investigate the expression of indoleamine 2,3-dioxygenase 1(IDO1)and kynureninase(KYNU)in oral squamous cell carcinoma(OSCC)and their relationship with pathological features and prognosis.Methods A total of 98 OSCC patients admitted to the hospital from January 2018 to June 2020 were enrolled in the study.OSCC tissue samples and corresponding adjacent tissue samples were collected.Immunohistochemical method was used to detect the expression of IDO1 and KYNU in tissues.The relationship between IDO1 and KYNU expression and the clinicopathological characteristics of OSCC patients was analyzed.Multivariate Cox regression analysis was used to analyze the factors affecting the prognosis of OSCC patients.Results The positive expression rates of IDO1 and KYNU in OSCC tissues were higher than those in adjacent tissues(P<0.05).The positive expression rates of IDO1 and KYNU in patients with poor differentiation,depth of invasion(DOI)>5 mm,lymph node metastasis,and clinical stageⅢ-Ⅳwere higher than those in patients with medium or high differentiation,DOI≤5 mm,no lymph node metastasis,and clinical stageⅠ-Ⅱ,respectively(P<0.05).The 3-year overall survival rates of OSCC patients with medium or high differentiation,DOI≤5 mm,no lymph node metastasis,clinical stageⅠ-Ⅱ,negative expression of IDO1,and negative expression of KYNU were higher than those of OSCC patients with low differentiation,DOI>5 mm,lymph node metastasis,clinical stageⅢ-Ⅳ,positive expression of IDO1,and positive expression of KYNU,respectively(P<0.05).Multivariate Cox regression analysis showed that DOI>5 mm(HR=3.225,95%CI:1.496-6.954),positive expression of IDO1(HR=3.714,95%CI:1.941-7.105),positive expression of KYNU(HR=4.150,95%CI:1.887-9.124)were the influencing factors of the prognosis of OSCC patients(P<0.05).Conclusion IDO1 and KYNU are highly expressed in cancer tissues of OSCC patients,which are closely related to the DOI,clinical stage,and differentiation degree of OSCC.IDO1 and KYNU are hopeful to be used as markers to assist in evaluating the prognosis of patients.