连翘叶提取物通过PXR/CYP17A1影响孕马血清促性腺激素在肝脏的代谢

Forsythia suspensa Leaves Extract Affects the Metabolism of Pregnant Mare Serum Gonadotropin in Liver through PXR/CYP17A1

【目的】研究连翘叶提取物(Forsythia suspensa leaves extract,FSLE)对孕马血清促性腺激素(pregnant mare serum gonadotropin,PMSG)在肝脏中代谢的影响,改善在畜牧生产中因PMSG代谢缓慢而造成的氧化应激。【方法】采用半仿生酶醇法提取连翘叶有效成分,给予昆明雌鼠不同含量的FSLE进行动物体内试验;收集小鼠血清,进行酶联免疫吸附试验,检测各组小鼠血清中PMSG的质量浓度;采集小鼠肝脏组织,检测氧化应激指标谷胱甘肽、超氧化物歧化酶、丙二醛和过氧化氢的水平;采用Western-blot检测小鼠肝脏组织孕烷X受体(pregnane X receptor,PXR)和细胞色素酶P45017A1(cytochrome P45017A1,CYP17A1)蛋白的表达水平。【结果】FSLE可显著降低小鼠血清中PMSG的质量浓度,且可以减缓PMSG代谢引起的氧化应激。Western-blot结果显示:FSLE可显著下调小鼠肝组织中PXR和CYP17A1蛋白的表达。【结论】连翘叶提取物可能是通过下调肝脏中PXR和CYP17A1的表达减缓PMSG代谢引起的氧化应激。

[Purpose]To study the effects of Forsythia suspensa leaves extract(FSLE)on the metabolism of pregnant mare serum gonadotropins(PMSG)in liver,improving the damage to the animal organism caused by the slow metabolism of PMSG in livestock production.[Methods]The active ingredient of forsythia leaves was extracted by semi-bionic enzyme alcohol method,and then the different contents of FSLE were given to female Kunming rats for in vivo animals experiment.The serum of mice was collected and enzyme-linked immunosorbent assay was performed to detect the mass concentration of PMSG in the serum of each group of mice.Liver tissues of mice were collected and the kit measured the levels of oxidative stress indicators,including glutathione,superoxide dismutase,malondialdehyde and hydrogen peroxide.Western-blot was used to detect the expression levels of pregnane X receptor(PXR)and cytochrome P45017A1(CYP17A1)proteins in mouse liver tissues.[Results]FSLE could significantly reduce the mass concentration of PMSG in the serum of mice,and it also might slow down the oxidative stress induced by PMSG metabolism.The results of Western-blot showed that FSLE could significantly down-regulate the expression levels of PXR and CYP17A1 in liver tissue.[Conclusion]FSLE may attenuate oxidative stress induced by PMSG metabolism through down-regulating the expression of PXR and CYP17A1 in liver tissue.