固相萃取-液相色谱法测定减肥茶中番泻苷A、番泻苷B的含量

Determination of sennoside a and sennoside B in weight-reducing tea by solid phase extraction combined with HPLC

目的建立固相萃取(solid phase extraction,SPE)-液相色谱法(high performance liquid chro-matography,HPLC)测定减肥茶中番泻苷A、番泻苷B含量的方法。方法样品经体积分数为70%的甲醇超声提取后,通过阴离子固相萃取小柱净化后,用体积分数2%氨水甲醇洗脱,洗脱液氮吹浓缩后得供试液,采用BDS HYPERSIL C18色谱柱分离,以甲醇-体积分数01%磷酸为流动相进行梯度洗脱,经二极管阵列检测器分析,外标法定量。结果番泻苷A和番泻苷B在1~100μgmL^(-1)质量浓度范围内线性良好,平均加标回收率为922%~984%(n=6);RSD为08%~33%(n=6)。结论本方法适用于减肥茶中番泻苷A、番泻苷B含量的检测。

Objective An analytical method based on solid phase extraction combined with high performance liquid chromatography was established for the determination of Sennoside A and Sennoside Bin weight-reducing tea.Methods After supersonic extractionwith 70%(volume fraction)methanolas solvate,the extracts were purified by anion-exchange solid phase column chromatography,eluted with methanol containing 2%(volume fraction)ammonia,and concentrated in a stream of nitrogen to obtain the final sample solutions.These samples were separated with a BDS HYPERSIL C18 chromatographic column,using methanol and 0.1%phosphoric acid(volume fraction)as the mobile phase,analyzed using a diode array detector and quantified with reference to an external standard.Results Sennoside A and SennosideBhad good linearity in the mass concentration range of 1-100μgmL^(-1),quantitative limits were 10 mg100 g^(-1).The average recoveries were in the range from 922%to 984%(n=6),relative standard deviations were in the range of 08%-33%(n=6).Conclusion This method accordingly proved to be suitable for the determination of Sennoside A and Sennoside Bin weight-reducing tea.