文冠果花中具有抗AD活性三萜的定性与定量分析

Qualitative and quantitative analysis of triterpenoids with anti-AD activity in flowers of Xanthoceras sorbifolia Bunge

目的建立UPLC-QTOF-MS法对文冠果花中8种三萜类化合物进行定性定量分析。方法采用ACQUITY UPLC HSS T3 C18色谱柱(100 mm×2.1 mm,1.8μm),以体积分数0.1%甲酸水为流动相A,乙腈为流动相B,梯度洗脱,流速0.4 mL·min^(-1),电喷雾离子源(ESI),在正、负离子模式下进行分析。结果通过与对照品的色谱和质谱数据进行比较,在文冠果花中共鉴定了8个三萜类化合物的结构;在同一实验条件下,可以完成8个组分的定量分析,方法学验证均符合要求,定量测定结果表明,与花(330.6μg·g^(-1))相比,花苞中三萜类化合物的含量较高(384.5μg·g^(-1))。结论所建立的UPLC-QTOF-MS方法适用于文冠果花中三萜类化合物的定性与定量分析,为文冠果花的合理应用和质量控制提供了理论依据。

Objective To establish an UPLC-QTOF-MS method for the qualitative and quantitative analysis of 8 triterpenoids in the flower of Xanthoceras sorbifolium Bunge.Methods The mobile phase was performed on an ACQUITY UPLC HSS T3 C_(18)column(100 mm×2.1 mm,1.8μm)with 0.1%formic acid as mobile phase A and acetonitrile as mobile phase B by gradient elution.The flow rate was 0.4 mL·min~(-1).The analysis was performed in positive ion mode and negative ion mode with electrospray ionization source(ESI).Results By comparing with the retention time and MS data of the reference compounds,the structures of 8 triterpenoids were identified in the flowers of Xanthoceras sorbifolium Bunge.Under the same experimental conditions,the quantitative analysis of 8 components was completed.And the developed quantitative method of 8 triterpenoids was verified to be precise and accurate.The results showed that the content of 8 triterpenoids in buds(384.5μg·g~(-1))was higher than that in flowers(330.6μg·g~(-1)).Conclusion The established UPLC-QTOF-MS methods are suitable for the qualitative and quantitative analysis of triterpenoids in flowers of Xanthoceras sorbifolium Bunge.This research provides a theoretical basis for the rational application and quality control of the flowers of Xanthoceras sorbifolium Bunge.