安肠止泻方剂联合针刺治疗腹泻型肠易激综合征模型大鼠对炎症因子的效果

Effect of Anchang Antidiarrheal Prescription Combined with Acupuncture on Inflammatory Factors in Rats with Diarrheal Irritable Bowel Syndrome

目的:分析安肠止泻方剂联合针刺治疗腹泻型肠易激综合征(IBS-D)模型大鼠对炎症因子的效果。方法:50只SPF级SD大鼠(2周龄)分为健康组(未造模,日常灌胃生理盐水)、模型组(造模成功后不予其他干预,日常灌胃生理盐水)、方剂组(造模成功后予以安肠止泻方剂)、针刺组(造模成功后予足三里、关元、内关穴针刺干预)和联合组(联合使用方剂组、针刺组干预方式),干预周期均为14 d。比较各组造模前和造模后(造模14 d)、干预前和干预后(干预14 d)白介素1(IL-1)、白介素6(IL-6)、肿瘤坏死因子α(TNF-α)含量的统计学差异。结果:造模后,模型组IBS-D大鼠IL-1、IL-6、TNF-α含量均高于健康组,差异均有统计学意义(P<0.05)。组内比较发现,方剂组、针刺组、联合组IBS-D大鼠干预后的IL-1、IL-6、TNF-α含量均低于干预前,差异均有统计学意义(P<0.05)。干预后组间比较,除联合组针刺组TNF-α含量、针刺组和方剂组IL-6和TNF-α含量的差异无统计学意义(P>0.05),其余各组IL-1、IL-6、TNF-α含量差异均有统计学意义(P<0.05)。结论:安肠止泻方剂联合针刺干预IBS-D效果优于单种干预措施,能明显控制体内炎症状态。

Ojective:To analyze the effect of Anchang Antidiarrheal Prescription combined with acupuncture on inflammatory factors in rats with diarrheal irritable bowel syndrome(IBS-D).Methods:Fifty SPF SD rats(2 weeks old)are divided into healthy group(no modeling,daily gavage of normal saline),model group(no other intervention after successful modeling,daily gavage of normal saline),prescription group(after successful modeling,Anchang Antidiarrhea Prescription is given),acupuncture group(after successful modeling,acupuncture intervention is given at Zusanli,Guanyuan and Neiguan points),and combined group(intervention methods of combined prescription group and acupuncture group),the intervention period is 14 days.The contents of interleukin-1(IL-1),interleukin-6(IL-6)and tumor necrosis factorα(TNF-α)are compared between groups before and after modeling(14 d),before and after intervention(14 d).Results:After modeling,the contents of IL-1,IL-6 and TNF-αin IBS-D rats in model group are higher than those in healthy group,and the differences are statistically significant(P<0.05).Intra-group comparison shows that the contents of IL-1,IL-6 and TNF-αof IBS-D rats in prescription group,acupuncture group and combined group are lower after intervention than before intervention,with statistical significance(P<0.05).Postintervention comparisons between groups,the contents of TNF-αin combination group and acupuncture group,and IL-6 and TNF-αin acupuncture group and prescription group have no statistical significance(P>0.05),and the contents of IL-1,IL-6 and TNF-αin other groups have statistical significance(P<0.05).Conclusions:The effect of Anchang Antidiarrheal Prescription combined with acupuncture in the intervention of IBS-D is better than that of single intervention,and can obviously control the inflammation in vivo.