枸杞提取物中玉米黄质和叶黄素含量的高效液相测定方法

Determination of Zeaxanthin and Lutein in Lycium Barbarum Extract by HPLC

选用色谱柱Innoval ODS-2(4.6 mm×250 mm,5µm),以0.05%三乙胺-乙腈为流动相,流速为0.8 mL/min,柱温为28℃,在450 nm波长处测定样品。结果显示:玉米黄质在0.207~20.7µg/mL范围内线性关系良好,判定系数r^(2)=1.0000,平均回收率为113.72%(n=6),RSD为1.50%,检出限为0.027µg/mL;叶黄素在0.216~21.6µg/mL范围内线性关系良好,判定系数r^(2)=1.0000,平均回收率为105.96%(n=6),RSD为3.78%,检出限为0.056µg/mL。该方法快速、简单稳定、结果准确,适用于同时测定枸杞提取物中叶黄素和玉米黄质。

The chromatographic column Innoval ODS-2(4.6 mm×250 mm,5µm)is selected with 0.05%triethylamine-acetonitrile as the mobile phase at a flow rate of 0.8 mL/min and a column temperature of 28℃.The samples are determined at 450 nm.The linear relationship of zeaxanthin is good within the range of 0.207 to 20.7µg/mL,with a determination coefficient R^(2)=1.0000,an average recovery rate of 113.72%(n=6),a RSD of 1.50%,and a detection limit of 0.027µg/mL.The linear relationship of lutein is good within the range of 0.216 to 21.6µg/mL,with a determination coefficient R^(2)=1.0000,an average recovery rate of 105.96%(n=6),a RSD of 3.78%,and a detection limit of 0.056µg/mL.The method is fast,simple,stable and accurate.It can be used for simultaneous determination of lutein and zeaxanthin in Lycium Barbarum extract.