基于cyt b基因分析河南省4大水系红鳍原鲌种群的遗传多样性

Genetic Diversity Analysis of Cultrichthys erythropterus in Four River Systems in Henan Province Based on cyt b Gene

选用长江、黄河、淮河、海河4大水系的红鳍原鲌Cultrichthys erythropterus样本共100尾,基于cyt b基因分析其种群的遗传多样性、遗传结构及种群历史动态等。结果显示:河南省4大水系红鳍原鲌种群整体单倍型多态性为0.909,整体核苷酸多样性为0.004 88,具有较高的遗传多样性;4个水系均有特有单倍型,这表明红鳍原鲌种群的遗传分化数值较大,遗传分化程度较高;通过构建和分析系统发育树、贝叶斯系统发生树和单倍型网络图,发现河南省4大水系红鳍原鲌种群间谱系分化不明显,且无明显的地理结构;AMOVA分析显示,种群内的变异占72.11%,组间的变异占4.63%,表明变异主要发生在种群内;Tajima’s D和Fu’s F_S分析结果表明,河南省红鳍原鲌种群近期未发生过种群扩张事件。本文研究和分析了河南省境内红鳍原鲌种群遗传多样性和遗传结构,为河南省红鳍原鲌种质资源的保护和合理开发利用提供了参考。

In order to explore the genetic diversity and analyze the genetic structure of Cultrichthys erythropterus popula-tion in complex river systems in Henan Province,100 samples of C.erythropterus were collected from the four major river systems of the Yangtze River,the Yellow River,the Huaihe River and the Haihe River.The genetic diversity,genetic structure and historical dynamics of the population were analyzed based on mitochondrial cyt b gene.The results showed that the haplotype polymorphism(Hd)and nucleotide diversity(π)were 0.909 and 0.00488,respectively,indicating high genetic diversity of the population.Unique haplotypes were identified in the four river systems,this indicated that the genetic differentiation between C.erythropterus populations is relatively big and the degree of genetic differentiation is rela-tively high.Phylogenetic trees,Bayesian phylogenetic trees and haplotype network maps were constructed and analyzed.It was found that there was no obvious phylogenetic differentiation and geographical structure among C.erythropterus popula-tions in the four river systems of Henan Province.AMOVA analysis showed that the variation within populations accounted for 72.11%,and the variation among populations accounted for 4.63%,indicating that the variation mainly occurred within populations.The results of Tajima’s D and Fu’s FS analysis showed that there was no recent population expansion event of C.erythropterus in Henan Province.This study provides reference for the protection and rational development of the germplasm resources of C.erythropterus.