小鼠甲状腺功能减退模型的建立

Establishment of hypothyroidism model in mice

目的将C57BL/6和KM小鼠甲状腺通过两种不同术式全切后,验证甲状腺功能减退模型造模是否成功,同时比较不同术式造模的成功率差异。方法本研究采用C57BL/6和KM小鼠进行结扎法(术式Ⅰ)和止血法(术式Ⅱ)进行甲状腺全切操作,并记录操作详细过程,利用酶联免疫吸附法检测、对比术前及术后小鼠的血清TT3、TT4及TSH水平、体重及颈部组织苏木精-伊红(HE)染色验证模型。结果两种术式均造成两种属模型组小鼠血清TT3、TT4降低(P<0.05),TSH升高(P<0.001)。术式Ⅰ组和术式Ⅱ组小鼠术后28 d内存活率分别为C57BL/6小鼠:40%和60%;KM小鼠:50%和40%。两种属手术组小鼠术后体重较假手术组均明显升高。HE染色和显微镜观察结果表明,两种属小鼠颈部切取组织为甲状腺组织,且离体后甲状腺后背膜完整。结论两种手术方式均能造成两种属小鼠甲状腺功能减退,但需要熟悉小鼠甲状腺及周围组织解剖关系,提高操作熟练度,预防术后低钙及感染从而提高造模后小鼠存活率。

Objective Total thyroidectomy of C57BL/6 and KM mice was performed by two different surgical methods to verify the success of mouse hypothyroidism model modeling,and compared the success rate of different surgical methods.Methods C57BL/6 and KM mice underwent total thyroidectomy by ligation(operation methodⅠ)or hemostasis(operation methodⅡ),and the detailed operation processes were recorded.Serum TT3,TT4 and TSH levels detected by enzyme-linked immunosorbent assay,body weight,and hematoxylin-eosin(HE)-stained neck tissue were compared before and after surgery to verify the model.Results Serum TT3 and TT4 levels were decreased(P<0.05)and TSH was increased(P<0.001)in both model groups.The 28-day postoperative survival rates were 40%and 60%in groupsⅠandⅡ,respectively,and 50%and 40%in KM mice.Body weights were significantly higher in both model groups compared with the sham control group.HE staining and microscopic observation showed that the cervical tissue in both strains was thyroid tissue,and the back membrane of the thyroid remained intact after isolation.Conclusions Both surgical method can induce hypothyroidism in C57BL/6 and KM mice;however,it is necessary to consider the anatomical relationship of the thyroid gland to the surrounding tissue,improve the proficiency of the surgical operation,prevent the occurrence of postoperative hypocalcemia and infection,and thus improve the survival rate of the model mice.