海南西沙试验站大气霉菌群落初步调查

Preliminary Investigation of Atmospheric Fungal Community in Xisha Experimental Station

目的 筛选西沙试验站大气环境优势菌种,进行西沙试验站装备敏感菌研究。方法 采用撞击法进行大气霉菌采样,用统计学法计算出不同试验场地真菌数量,结合形态学法与ITS1-5.8S-TIS2或26SrDNA D1/D2区序列分析法鉴定菌种,并用SPSS初步探讨大气真菌与环境因素的关系,分析西沙试验站装备敏感菌。结果 西沙试验站优势种为Aspergillus aculeatus、Penicillium citrinum、Pithomyces sacchar、变红镰孢霉Fusarium incarnatum、Cladosporium oxysporum和Cladosporium oryzae,影响西沙试验站大气霉菌群落结构的重要环境因素是相对湿度和风速,贮存在西沙试验站的装备容易长霉,多种材料有不同的敏感菌,尤其是涂层样件和橡胶类材料。结论 西沙试验站大气环境中存在种类繁多的霉菌。

The work aims to screen dominant fungal species in the atmospheric environment of Xisha Experimental Station and conduct research on sensitive fungal in Xisha Experimental Station equipment.The impact method was used for sampling atmospheric fungi,and statistical methods were used to calculate the number of fungi in different experimental sites.Morphological methods were used,the species were identified in combination with ITS1-5.8S-TIS2 or 26S rDNA D1/D2 region sequence analysis.SPSS was used to preliminarily explore the relationship between atmospheric fungi and environmental factors,and to analyze the sensitive fungi in Xisha Experimental Station equipment.The dominant species at the Xisha Experimental Station were Aspergillus aculatus,Penicillium citrinum,Pithomyces sacchar,Fusarium incarnatum,Cladosporium oxysporum,and Cladosporium oryzae.The important environmental factors affecting the structure of the atmospheric mold community at Xisha Experimental Station were relative humidity and wind speed.Equipment stored in Xisha Experimental Station was prone to mold growth,and various materials had different sensitive fungi,especially for coating samples and rubber materials.There are various types of fungi in the atmospheric environment of Xisha Experimental Station.