实时荧光PCR检测在支原体肺炎诊断中的应用价值分析

Analysis the Application Value of Real-time Fluorescence PCR Test in the Diagnosis of Mycoplasma Pneumoniae

目的探讨在支原体肺炎诊断中选择实时荧光聚合酶链式反应(Polymerase Chain Reaction,PCR)检测的价值,并分析该方案的诊断效能。方法选取2022年8月—2023年10月来宾市人民医院收治的1200例疑似支原体肺炎患儿为研究对象,所有患儿均采集血清和呼吸道分泌物,用化学发光法测定血清中肺炎支原体抗体,用实时荧光PCR法检测呼吸道分泌物中的肺炎支原体核酸(Mycoplasma Pneumoniae Nucleic Acid,MP-DNA),以病原学检查结果为金标准,对比各个方案的诊断效能,分析不同年龄段患儿的肺炎支原体DNA检测结果。结果实时荧光PCR检测灵敏度(99.36%)、特异度(98.08%)、准确度(99.08%)、阳性预测值(99.36%)、阴性预测值(98.08%)高于MP血清学方法检测,差异有统计学意义(χ^(2)=16.312、7.686、23.788、7.595、16.119,P均<0.05)。结论在支原体肺炎诊断中应用实时荧光PCR检测方案可提高准确率,同时该方案具有早期、快速、简便等优势,可为临床医师制定治疗方案提供参考。

Objective To explore the value of selecting quantitative real-time fluorescence polymerase chain reaction(PCR)testing in the diagnosis of Mycoplasma pneumonia and to analyze the diagnostic efficacy of this protocol.Methods 1200 children with suspected mycoplasma pneumonia admitted to Laibin People′s Hospital from August 2022 to October 2023 were selected as the study objects.Serum and respiratory secretions were collected from all the children.Antibodies to Mycoplasma pneumoniae in serum were determined by chemiluminescence,and Mycoplasma pneumoniae nucleic acid(MP-DNA)in respiratory secretions was detected by real-time fluorescence PCR.The results of Mycoplasma pneumoniae DNA were analyzed in children of different ages by comparing the compliance rate and diagnostic efficacy of each protocol using the results of pathogenicity examination as the gold standard.Results The sensitivity(99.36%),specificity(98.08%),accuracy(99.08%),positive predictive value(99.36%),and negative predictive value(98.08%)of real-time fluorescent PCR detection were higher than those of MP serologic method detection,and the differences were statistically significant(χ^(2)=16.312,7.686,23.788,7.595,16.119,all P<0.05).Conclusion The application of real-time fluorescence PCR test protocol in the diagnosis of Mycoplasma pneumonia can improve the accuracy rate,and at the same time,the protocol has the advantages of early,rapid and simple,which can provide a reference for clinicians to develop the treatment plan.