CLIA法检测性激素六项用于女性生殖内分泌疾病诊断中的临床价值

Clinical Value of Detecting Six Sex Hormones by CLIA in the Diagnosis of Female Reproductive Endocrine Diseases

目的研究化学发光免疫测定(Chemiluminescent Immunoassay,CLIA)法检测性激素六项用于女性生殖内分泌疾病诊断中的临床价值。方法选取2021年2月—2023年2月于连云港市赣榆区妇幼保健院就诊的41例女性生殖内分泌疾病患者为观察组,并选取同时期41例女性健康体检者为对照组。采用CLIA法检测性激素六项,比较两组的性激素水平,并分析观察组在不同月经周期的性激素水平变化情况。结果观察组的卵泡生成素(Follicle Stimulating Hormone,FSH)、促黄体生成素(Luteinizing Hormone,LH)、泌乳素(Prolactin,PRL)、睾酮(Testosterone,T)水平分别为(22.15±3.21)mIU/mL、(47.32±4.36)mIU/mL、(185.36±3.51)ng/mL、(0.79±0.11)ng/mL,高于对照组的(13.50±2.17)mIU/mL、(6.27±1.54)mIU/mL、(13.22±2.62)ng/mL、(0.24±0.04)ng/mL,雌二醇(Estradiol,E2)、孕酮(Progesterone,P)水平分别为(10.43±2.35)pg/mL、(0.05±0.01)ng/mL,低于对照组的(22.22±4.64)pg/mL、(0.33±0.05)ng/mL,差异有统计学意义(t=14.295、56.845、251.651、30.088、14.515、35.161,P均<0.001)。观察组在不同月经周期,其性激素水平呈周期性变化,FSH、LH在卵泡期较低,前者在排卵期略微提高,但差异无统计学意义(P>0.05),而后者则有明显升高趋势,两者在黄体期则明显下降,差异有统计学意义(P均<0.05);PRL、T、P水平呈上升趋势,差异有统计学意义(P均<0.05);E2水平随着卵泡的发育不断升高,黄体期迅速降低,差异有统计学意义(P均<0.05)。结论CLIA法检测性激素六项用于女性生殖内分泌疾病诊断具有明显作用,可为临床治疗提供一定的指导作用。

Objective To investigate the clinical value of chemiluminescent immunoassay(CLIA)for detecting six sex hormones in the diagnosis of female reproductive endocrine diseases.Methods A total of 41 female patients with reproductive endocrine diseases who were treated in Ganyu District Maternal and Child Health Hospital of Lianyungang City from February 2021 to February 2023 were selected as the observation group,and 41 female health examination subjects during the same period were selected as the control group.Six items of sex hormones were detected by CLIA method,the sex hormone levels of the two groups were compared,and the changes of sex hormone levels in the observation group in different menstrual cycles were analyzed.Results The levels of follicle stimulating hormone(FSH),luteinizing hormone(LH),prolactin(PRL),and testosterone(T)in the observation group were(22.15±3.21)mIU/mL,(47.32±4.36)mIU/mL,(185.36±3.51)ng/mL,and(0.79±0.11)ng/mL,respectively,which were higher than those in the control group[(13.50±2.17)mIU/mL,(6.27±1.54)mIU/mL,(13.22±2.62)ng/mL,(0.24±0.04)ng/mL],which were the levels of estradiol(E2)and progesterone(P)were(10.43±2.35)pg/mL and(0.05±0.01)ng/mL,respectively,lower than the control group′s(22.22±4.64)pg/mL and(0.33±0.05)ng/mL,the difference was statistically significant(t=14.295,56.845,251.651,30.088,14.515,35.161,all P<0.001).In the observation group,the levels of sex hormones showed periodic changes in different menstrual cycles,and the levels of FSH and LH were lower in the follicular phase,the former slightly increased in the ovulation phase,but the difference was not statistically significant(P>0.05),while the latter showed a significant increase trend,and the two significantly decreased in the luteal phase,the difference was statistically significant(both P<0.05),the levels of PRL,T and P were increased,and the differences were statistically significant(all P<0.05).E2 levels increased with the development of follicles,and decreased rapidly in luteal phase,and the differences were statistically significant(both P<0.05).Conclusion The detection of six sex hormones by CLIA has obvious effect on the diagnosis of female reproductive endocrine diseases,and can provide certain guidance for clinical treatment.