ASXL3基因在前列腺癌中的表达

The expression of ASXL3 in prostate cancer

目的探讨ASXL3基因在前列腺癌中的表达变化及其临床意义。方法从TCGA、GEPIA2、STRING和TIMER等多个生物信息学数据库获取数据,使用R软件或在线工具分析ASXL3 mRNA的表达变化,及其与前列腺癌患者预后、临床特征以及免疫细胞浸润之间的关系,并对ASXL3可能相互作用的基因以及表达相关基因进行GO富集和KEGG通路分析。利用qRT-PCR验证ASXL3 mRNA在前列腺癌细胞系中的表达情况。结果前列腺癌组织中ASXL3 mRNA的表达水平较正常组织显著降低,ASXL3 mRNA高表达与患者较好的总生存期相关,且ASXL3 mRNA表达与多种免疫细胞浸润正相关。STRING数据库分析显示ASXL3蛋白可能与BAP1、EZH2、ASXL2和BRD4等相互作用。与人正常前列腺基质细胞WPMY-1相比,ASXL3 mRNA在前列腺癌细胞系(LNCaP、C4-2、PC-3、DU145)中显著低表达。结论ASXL3在前列腺癌组织中表达下调,且与患者总生存期密切相关,有望成为前列腺癌预后生物标志物和潜在治疗靶点。

Objective To explore the expression and clinical significance of ASXL3 in prostate cancer.Methods The data were obtained from multiple bioinformatics databases,including TCGA,GEPIA2,STRING and TIMER.R or online tools were used to analyze the expression of ASXL3 mRNA and its association with the prognosis,clinical features,and immune cell infiltration in prostate cancer.GO and KEGG pathway enrichment analyses were conducted using ASXL3 interacting proteins or expression-related genes.The expression of ASXL3 mRNA in prostate cancer cell lines was verified by using qRT-PCR.Results The expression level of ASXL3 mRNA in prostate cancer tissues was significantly lower compared to normal tissues.The high expression of ASXL3 mRNA was related to the better overall survival of prostate cancer patients,and the expression of ASXL3 mRNA was positively correlated with the infiltration of various immune cells.STRING database analysis revealed potential interactions of ASXL3 with BAP1,EZH2,ASXL2,and BRD4.ASXL3 mRNA expression was significantly lower in prostate cancer cell lines(LNCaP,C4-2,PC-3,DU145)than normal prostate stromal cell WPMY-1.Conclusion ASXL3 is down-regulated in prostate cancer tissues and is associated with overall survival,which may provide a novel prognostic biomarker and potential therapeutic target for prostate cancer.