急性脑梗死患者血清miR-210和miR-940水平及其临床意义

Levels and clinical significance of serum miR-210 and miR-940 in patients with acute cerebral infarction

目的探讨急性脑梗死患者血清微小RNA(miR)-210和miR-940的表达水平及其临床意义。方法选取绵阳市中心医院2021年9月—2022年9月收治的80例急性脑梗死患者作为本研究的研究组,依据美国国立卫生研究院卒中量表(NIHSS)评分标准分为重型患者(15例)、中型患者(35例)、轻型患者(30例),并以同期体检正常的50例健康人员作为对照组,收集各组一般资料,采用qRT-PCR法检测各组血清miR-210和miR-940水平;ROC曲线分析血清miR-210和miR-940水平对急性脑梗死的诊断价值;Pearson法分析急性脑梗死患者血清中miR-210和miR-940水平的相关性;Logistics回归分析影响NIHSS评分升高的因素。结果在糖尿病、收缩压及舒张压的比较中,对照组及研究组具有统计学差异(P<0.05),但在年龄、性别、总胆固醇、甘油三酯以及吸烟史、饮酒史的比较中均无统计学差异(P>0.05);与对照组相比,研究组患者血清中miR-210和miR-940水平显著下降(P<0.05);与轻度急性脑梗死患者相比,重度、中度患者血清中miR-210、miR-940水平显著下降;与中度急性脑梗死患者相比,重度患者血清中miR-210、miR-940水平显著下降(P<0.05);Pearson法分析显示miR-210和miR-940水平呈正相关。血清miR-210与miR-940水平以及二者联合诊断急性脑梗死患者的敏感度分别为88.75%、82.50%、80.00%,特异度分别为68.00%、80.00%、92.00%,曲线下面积分别为0.830、0.881、0.926。Logistics回归分析显示血清miR-210、miR-940水平为NIHSS评分升高的独立危险因素(P<0.05)。结论急性脑梗死患者血清中miR-210、miR-940水平显著下降,与患者病情程度有关,二者表达呈正相关,对急性脑梗死具有一定的诊断价值,二者联合诊断效能更佳。

Objective To investigate the expression levels and clinical significance of serum microRNA-210 and miR 940 in patients with acute cerebral infarction.Methods Eighty patients with acute cerebral infarction admitted to our hospital from September 2021 to September 2022 were selected as the study group of this study,according to the National Institutes of Health Stroke Scale(NIHSS),they were grouped into severe patients(15 cases),moderate patients(35 cases)and mild patients(30 cases),and 50 healthy people with normal physical examination in the same period were taken as the control group.General information of each group was collected,the serum levels of miR-210 and miR-940 were measured by qRT-PCR;ROC curve was applied to analyze the diagnostic value of serum miR-210 and miR-940 levels for acute cerebral infarction;Pearson's method was applied to analyze the correlation between the levels of miR-210 and miR-940 in the serum of patients with acute cerebral infarction;and Logistic regression was applied to analyze the factors affecting the increase of NIHSS score.Results In the comparison of diabetes,systolic blood pressure and diastolic blood pressure,there was a statistical difference between the control group and the study group(P<0.05),however,there was no statistical difference in age,sex,total cholesterol,triglyceride,smoking history and drinking history(P>0.05);compared with the control group,the serum levels of miR-210 and miR-940 in the study group were obviously decreased(P<0.05);compared with patients with mild acute cerebral infarction,the levels of miR-210 and miR-940 in serum of patients with severe and moderate cerebral infarction decreased obviously;compared with patients with moderate acute cerebral infarction,the levels of miR-210 and miR-940 in serum of severe patients were obviously decreased(P<0.05);Pearson analysis showed that there was a positive correlation between miR-210 and miR-940 levels.The sensitivity of serum miR-210 and miR-940 levels and their combination in the diagnosis of patients with acute cerebral infarction was 88.75%,82.50%and 80.00%respectively,the specificity was 68.00%,80.00%and 92.00%respectively,and the area under the curve was 0.830,0.881 and 0.926 respectively.Logistics regression analysis showed that serum miR-210 and miR-940 levels were independent risk factors for increased NIHSS score(P<0.05).Conclusion The levels of miR-210 and miR-940 in the serum of patients with acute cerebral infarction decrease obviously,which is related to the degree of disease of the patients.The expression of miR-210 and miR-940 is positively correlated,which has certain diagnostic value for acute cerebral infarction.The combined diagnosis of the two is better.