龙岩市不同来源副溶血性弧菌耐药性分析

Analysis of drug resistance of vibrio parahaemolyticus from different sources in Longyan city

目的了解福建省龙岩市腹泻患者粪便标本和市售水产品的副溶血性弧菌(VP)的耐药特征。方法收集VP162株,其中人源32株,水产品来源130株,以实时荧光定量PCR方法检测毒力基因,判断产毒株和非产毒株,采用微量肉汤稀释法开展16种药物敏感试验。结果162株不同来源的VP对阿莫西林、环丙沙星、左氧氟沙星、四环素、多西环素、氯霉素和阿奇霉素的敏感率均为100.0%,但对9种药物出现不同程度的耐药,耐药率较高的是头孢唑林(25.3%)、氨苄西林(15.4%)和复方磺胺甲恶唑(13.0%);产毒株与非产毒株耐药率差异无统计学意义(χ^(2)=0.353,P>0.05);共有7种耐药谱,耐一种药物的耐药率为15.4%,3种及以上多重耐药率为13.0%。结论腹泻患者VP与市售水产品VP的耐药特征接近,应严格控制抗生素在临床和养殖业中的使用。

Objective To analyze the characteristics of drug resistance of vibrio parahaemolyticus(VP)in fecal samples of diarrhea patients and market aquatic products in Longyan city.Methods Out of 162 strains of VP,there were 32 strains from human sources and 130 strains from aquatic products.The virulence genes were detected with real-time fluorescence quantitative PCR method to determine the toxigenic strains and non-toxigenic strains.The micro broth dilution method was used for 16 drug sensitivity tests.Results The sensitivity rates of amoxicillin,ciprofloxacin,levofloxacin,tetracycline,doxycycline,chloramphenicol and azithromycin were 100.0%in 162 strains of VP from different sources.But there were varying degrees of drug resistance to 9 drugs.The resistance rates of cefazolin(25.3%),ampicillin(15.4%),and compound sulfamethoxazole(13.0%)were high.There was no statistical difference in drug resistance rates between toxigenic strains and non-toxigenic strains(χ^(2)=0.353,P>0.05).There were 7 drug resistant spectrum.The rate of resistance to one drug was 15.4%,and the rate of multiple resistance to three drugs and more was 13.0%.Conclusions The characteristics of drug resistance of VP are similar in diarrhea patients and commercially available aquatic products,and the antibiotics used in clinical and aquaculture should be strictly controlled.