响应面法优化异麦芽酮糖结晶工艺

Optimization of isomaltulose crystallization process using response surface methodology

以蔗糖为原料,经蔗糖异构酶转化得到异麦芽酮糖、蔗糖和海藻酮糖的混合物,通过结晶得到异麦芽酮糖晶体。以异麦芽酮糖结晶收率为指标,通过单因素实验考察晶种添加量、补料流速和降温频率对收率的影响,再采用响应面法优化结晶工艺。结果表明,晶种添加量和补料流速对异麦芽酮糖结晶收率的影响更为显著。响应面法优化得到的最佳工艺为:晶种添加质量为体系质量的0.34%,补料流速为0.21L/h,降温频率为0.95℃/h,此条件下异麦芽酮糖结晶收率为91.06%。

Using sucrose as the raw material,a mixture of isomaltulose,sucrose,and trehalose was obtained through sucrose isomerase conversion,and isomaltulose crystals were obtained through crystallization.Using the yield of isomaltulose crystallization as an indicator,the effects of seed addition,feed flow rate and cooling frequency on the yield were investigated by single factor experiment,and the crystallization process was optimized by response surface method.The results showed that the yield of isomaltolose crystallization was significantly affected by the amount of seed and feed flow rate.The optimal process obtained by response surface methodology was as follows:the seed crystal quality was 0.34%of the system quality,feed flow rate 0.21L/h,cooling frequency 0.95℃/h.Under these conditions,the crystallization yield of isomaltolose was 91.06%.