miR-205和张力蛋白同源缺失基因在食管癌组织中的表达及与根治术后预后的关系

Expression of miR-205 and chromosome 10 phosphatase and tensin homologous deletion gene in esophageal carcinoma and their relationship with prognosis after radical resection

目的探讨miR-205与10号染色体磷酸酶和张力蛋白同源缺失基因(PTEN)在食管癌组织中的表达及与根治术后预后的关系。方法2018年9月~2019年9月收治的食管癌病人78例均行根治术,收集癌旁组织和癌组织标本,采用荧光定量PCR法检测癌旁组织和癌组织miR-205、PTEN表达,分析食管癌病人癌组织miR-205、PTEN表达水平与病理特征的关系,所有病人随访至2022年9月30日,采用Kaplan-Meier分析食管癌病人癌组织miR-205、PTEN表达与预后的关系。结果食管癌病人癌组织miR-205表达水平高于癌旁组织,PTEN表达水平低于癌旁组织,差异有统计学意义(P<0.05);食管癌病人癌组织miR-205表达水平与PTEN表达水平呈负相关(r=-0.397,P<0.001);淋巴结转移、低分化、Ⅲ~Ⅳ期食管癌病人癌组织miR-205表达水平分别高于无淋巴结转移、高中分化、Ⅰ~Ⅱ期病人,差异有统计学意义(P<0.05),PTEN表达水平分别低于无淋巴结转移、高中分化、Ⅰ~Ⅱ期病人,差异有统计学意义(P<0.05);所有病人随访3年,失访2例,死亡32例,存活44例,死亡病人癌组织miR-205表达水平高于存活病人,PTEN表达水平低于存活病人,差异有统计学意义(P<0.05);Kaplan-Meier分析显示,癌组织miR-205、PTEN高表达与低表达病人的生存曲线比较差异有统计学意义,癌组织miR-205低表达、PTEN高表达病人的生存率更高,差异有统计学意义(P<0.05)。结论食管癌病人癌组织miR-205、PTEN表达水平与预后密切相关,检测食管癌病人癌组织miR-205、PTEN表达水平,可作为预测预后的重要参考指标。

Objective To explore the expression of miR-205 and chromosome 10 phosphatase and tensin homologous deletion gene(PTEN)in esophageal carcinoma and their relationship with the prognosis after radical surgery.Methods 78 patients with esophageal cancer undergoing radical resection admitted to the hospital from September 2018 to September 2019 were selected.Their paracancerous tissue and cancerous tissue samples were collected.Fluorescent quantitative PCR was used to detect the expression of miR-205 and PTEN in adjacent and cancerous tissues.The relationship between the expression level of miR-205,PTEN and the pathological characteristics of esophageal cancer tissues was analyzed.All patients were followed up until September 30,2022.Kaplan Meier was used to analyze the relationship between the expression of miR-205,PTEN and the prognosis after radical resection of esophageal cancer.Results The expression level of miR-205 in cancer tissues was higher than that in adjacent tissues(P<0.05),and the expression level of PTEN was lower than that in adjacent tissues(P<0.05).The expression level of miR-205 was negatively correlated with that of PTEN(r=0.397,P<0.001).The expression level of miR-205 in cancer tissue of patients with lymph node metastasis,low differentiation,and stage Ⅲ to Ⅳ esophageal cancer was higher than that of patients without lymph node metastasis,high school differentiation,and stage Ⅰ to Ⅱ(P<0.05),and the expression level of PTEN was lower than that of patients without lymph node metastasis,high school differentiation,and stage Ⅰ to Ⅱ(P<0.05).All patients were followed for 3 years,a total of 32 patients died and 44 patients survived.The expression level of miR-205 in cancer tissues of the deceased patients was higher than that of the surviving patients(P<0.05),and the expression level of PTEN was lower than that of the surviving patients(P<0.05).Kaplan-Meier analysis showed that the difference of survival curve between patients with high and low expression of miR-205 and PTEN in cancer tissues was statistically significant,and patients with low expression of miR-205 and high expression of PTEN in cancer tissues had higher survival rates(P<0.05).Conclusion The expression levels of miR-205 and PTEN in cancer tissues of patients with esophageal cancer are closely related to the prognosis after radical surgery.The expression levels of miR-205 and PTEN in cancer tissues of patients with esophageal cancer were detected,which can be used as an important reference index to predict the prognosis after radical resection.