猴头菇多糖-纳米硒的制备、结构表征及其生物活性研究

Study on preparation,structural characterization and biological activity ofselenium nanoparticles conjugated with hericium erinaceus polysaccharides

以亚硒酸钠为硒源,利用微生物将无机硒转化为纳米硒(SeNPs),在此基础上,以猴头菇多糖为载体制备得到猴头菇多糖-纳米硒(HEPS-SeNPs).通过傅里叶变换红外光谱仪、X射线衍射仪、扫描电子显微镜、纳米粒度仪、透射电子显微镜等对纳米硒及猴头菇多糖-纳米硒进行结构表征;同时以SeNPs为对照,探究HEPS-SeNPs的抗氧化活性及对HepG_(2)细胞、PC12细胞、RAW264.7细胞细胞存活率的影响.结果表明:所制备的纳米硒及猴头菇多糖-纳米硒均为形貌规则、均一的球体,其元素组成均有Se元素检出;其中Se元素的含量高达671.42μg/g和169.63μg/g.傅里叶变换红外光谱表明HEPS主要通过O-H键等基团与纳米硒结合.与纳米硒相比,HEPS-SeNPs的粒径降低7.33%,电位绝对值提高了17.94%.同时,体外试验结果表明,SeNPs和HEPS-SeNPs对细胞均有一定的生物活性.当物质浓度大于0.4 mg/mL时,HEPS-SeNPs的生物活性高于SeNPs.且HEPS-SeNPs能提高抗氧化活性.研究结果为硒多糖的后续研究提供了理论依据.

Using sodium selenite as selenium source,the inorganic selenium was converted into selenium nanoparticles(SeNPs)by microorganisms.On this basis,selenium nanoparticles conjugated with hericium erinaceus polysaccharides(HEPS-SeNPs)was prepared by using hericium erinaceus polysaccharides as carrier.The structures of selenium nanoparticles and selenium nanoparticles conjugated with hericium erinaceus polysaccharides were characterized by fourier transform infrared spectroscopy,X-ray diffractometer,scanning electron microscope,nano-particle size analyzer and transmission electron microscope.Meanwhile,the antioxidant activity of HEPS-SeNPs and its effect on survival rate of HepG_(2)cells,PC12 cells and RAW264.7 cells were investigated with SeNPs as the control.The results showed that the prepared selenium nanoparticles and the selenium nanoparticles conjugated with hericium erinaceus polysaccharides were spheroids with regular and uniform morphology,and Se element was detected in the composition.The content of Se element is 671.42μg/g and 169.63μg/g.Fourier transform infrared spectroscopy shows that HEPS mainly binds to selenium nanoparticles through O-H bond and other groups.Compared with selenium nanoparticles,the particle size of HEPS-SeNPs is significantly reduced by 7.33%,and the absolute potential is increased by 17.94%.Both SeNPs and HEPS-SeNPs have certain biological activity on cells.When the concentration of HEPS-SeNPs was greater than 0.4 mg/mL,the biological activity of HEPS-SeNPs was higher than SeNPs.And HEPS-SeNPs can enhance antioxidant activity and other biological activities.This study provides a theoretical basis for the follow-up study of selenium polysaccharide.