基于网络药理学探讨健胃清肠合剂治疗急性胰腺炎作用机制

Exploration on the Mechanism of Jianwei Qingchang Mixture in Treating Acute Pancreatitis Based on Network Pharmacology

目的基于网络药理学探讨健胃清肠合剂治疗急性胰腺炎(AP)的作用机制。方法基于TCMSP、HERB数据平台检索,筛选健胃清肠合剂主要活性成分;在GeneCards、OMIM、DrugBank数据库获取AP相关疾病靶点,筛选出健胃清肠合剂活性成分对应靶点与AP的共同靶点;通过STRING数据库构建药物-疾病共同靶点的蛋白相互作用网络;利用DAVID平台进行GO功能和KEGG通路富集分析;采用Cytoscape3.9.1软件构建药物-成分-靶点-通路网络,应用网络拓扑学筛选健胃清肠合剂治疗AP的关键活性成分及核心靶点。结果获得健胃清肠合剂活性成分37个,包括大黄素、槲皮素、川陈皮素等,得到健胃清肠合剂防治AP靶点123个,核心靶点有PTGS2、HSP90AA1、PTGS1等,KEGG通路富集结果显示,健胃清肠合剂主要作用于IL-17、TNF、PI3K-Akt等多条信号通路。结论健胃清肠合剂可能通过槲皮素、β-谷甾醇、柚皮素、川陈皮素等活性成分作用于PTGS2、HSP90AA1、PTGS1等靶点,进而调控IL-17、TNF、PI3K-Akt、HIF-1等信号通路,以抑制炎症反应、抗氧化、调节自噬、减少细胞凋亡,多成分、多靶点、多通路治疗AP。

Objective To investigate the mechanism of Jianwei Qingchang Mixture in the treatment of acute pancreatitis(AP)based on network pharmacology.Methods Based on TCMSP and HERB data platforms,the main active components of Jianwei Qingchang Mixture were screened.AP related disease targets were retrieved from GeneCards,OMIM,and DrugBank databases,and common targets for the active components of Jianwei Qingchang Mixture and acute pancreatitis were screened out;protein interaction networks for drug-disease common targets was constructed through STRING Database;DAVID platform was used for GO function and KEGG pathway enrichment analysis;Cytoscape 3.9.1 software was used to construct a drug-active component-target-pathway network;network topology was used to screen key active components and core targets of Jianwei Qingchang Mixture in the treatment of AP.Results Totally 37 active components of Jianwei Qingchang Mixture were obtained,including emodin,quercetin,chuanchen retin,etc.,123 targets of Jianwei Qingchang Mixture in the treatment of AP were obtained,and the core targets were PTGS2,HSP90AA1,PTGS1,etc.,and the enrichment results of KEGG signaling pathway showed that Jianwei Qingchang Mixture mainly acted on IL-17 signaling pathway,TNF signaling pathway,PI3K-Akt and other signaling pathways.Conclusion Jianwei Qingchang Mixture may act on PTGS2,HSP90AA1,PTGS1 and other targets through active components such as quercetin,β-sitosterol,naringenin and chuanchen reticetin,and then regulate IL-17 signaling pathway,TNF signaling pathway,PI3K-Akt signaling pathway,HIF-1 signaling pathway to inhibit inflammatory response,antioxidation,regulate autophagy,affect apoptosis,and exert therapeutic effects on AP with multiple components,targets and pathways.