基于网络药理学的犀角地黄汤治疗川崎病和过敏性紫癜“异病同治”研究

Study on the“Different Diseases with the Same Treatment”of Kawasaki Disease and Henoch-Schonlein Purpura with Xijiao Dihuang Decoction Based on Network Pharmacology

目的采用网络药理学方法分析犀角地黄汤治疗川崎病和过敏性紫癜“异病同治”机制,为相关研究提供参考。方法通过TCMSP数据库检索犀角地黄汤活性成分及作用靶点,利用GeneCards、OMIM、DrugBank数据库检索川崎病和过敏性紫癜的靶点基因,通过STRING平台构建蛋白相互作用网络,应用Metascape平台分析靶点参与的生物学过程及作用通路,构建犀角地黄汤成分-川崎病和过敏性紫癜靶点-通路网络,最后进行分子对接验证。结果犀角地黄汤治疗川崎病和过敏性紫癜的核心成分为槲皮素、山柰酚、黄芩素等,核心靶点有IL6、AKT1等。犀角地黄汤治疗川崎病和过敏性紫癜的生物学通路主要作用于血管病变通路及炎症因子通路,其功能主要为调节细胞因子及信号受体的生命活动等。GO富集分析结果显示,交集靶点富集于细胞因子相互作用及信号相互作用。结论犀角地黄汤通过保护血管内皮、抗炎、调节免疫等作用达到川崎病和过敏性紫癜“异病同治”目的,其机制可能是槲皮素等通过抑制IL6、AKT1生成,阻止TNF信号通路激活及保护流体剪切力谱。

Objective To analyze the mechanism of“different diseases with the same treatment”of Kawasaki disease and Henoch-Schonlein purpura with Xijiao Dihuang Decoction using network pharmacology;To provide reference for related research.Methods The active components and action targets of Xijiao Dihuang Decoction were retrieved from TCMSP database.The target genes of Kawasaki disease and Henoch-Schonlein purpura were retrieved from GeneCards,OMIM and DrugBank databases,and the PPI network was constructed by STRING platform.The Metascape platform was used to analyze the biological processes and pathways involved in the targets,and then the network of“Xijiao Dihuang Decoction-Kawasaki disease and Henoch-Schonlein purpura target-pathway”was constructed.Finally,molecular docking was performed to verify the results.Results The core components of Xijiao Dihuang Decoction in the treatment of Kawasaki disease and Henoch-Schonlein purpura were quercetin,kakaferol,baicalein,etc.,and the core targets were IL6,AKT1,etc.The biological pathways of Xijiao Dihuang Decoction in the treatment of Kawasaki disease and Henoch-Schonlein purpura mainly acted on vascular disease pathway and inflammatory factor pathway,and its function was mainly to regulate the life activities of cytokines and signal receptors.GO enrichment analysis showed that the intersection targets were enriched in cytokine interaction and signal interaction.Conclusion Xijiao Dihuang Decoction can reach the goal of“different diseases with the same treatment”of Kawasaki disease and Henoch-Schonlein purpura by protecting vascular endothelium,antiinflammation,and regulating immunity.The mechanism is that quercetin and other substances inhibit the production of IL6 and AKT1,prevent the activation of TNF signaling pathway,and protect the fluid shear stress spectrum.