黄芪多糖对HD11鸡巨噬细胞转录组和代谢组的影响

Effects of Astragalus Polysaccharides on Transcriptome and Metabolome of HD11 Chicken Macrophages

旨在通过分析黄芪多糖对鸡巨噬细胞HD11转录组和代谢组的作用,探讨黄芪多糖(Astragalus polysaccharide, APS)对鸡巨噬细胞HD11的免疫调节机制。设置空白对照组(CONT组)和APS处理组(A50组),其中,A50组用含50μg·mL^(-1)APS的完全培养液培养HD11细胞12 h;CONT组用完全培养液培养HD11细胞12 h。采用代谢组学与转录组学技术分析CONT组与A50处理组细胞中差异基因和差异代谢物,并进行2个组学的联合分析。结果显示:在A50处理组与CONT组的转录组分析比较中,共检测到差异基因845个,其中上调基因415个,下调基因430个;差异表达基因主要富集在细胞因子-细胞因子受体相互作用、Toll样受体信号通路、吞噬体、AGE-RAGE等信号通路,其中,细胞因子-细胞因子受体相互作用、Toll样受体信号2条通路最为显著性;在A50处理组与CONT组的代谢组分析比较中,共筛选出差异代谢物89个,其中,下调差异代谢物为70个,上调的差异代谢物为19个。在A50处理组与CONT组的转录与代谢组联合分析中共同富集到可信度排名前十的通路有嘌呤代谢、碳代谢、磷酸戊糖途径、谷胱甘肽代谢、组氨酸代谢、氨基糖和核苷酸糖代谢、α同亚麻酸代谢、ABC转运体、铁死亡和酪氨酸代谢。APS可能是通过TLR2受体信号通路、糖酵解和磷酸戊糖途径的代谢重编程,进而发挥对HD11细胞的免疫调节作用。

This study aimed to explore the immunomodulatory mechanism of Astragalus polysaccharide(APS)on HD11 in chicken macrophages by analyzing the effects of APS on the transcriptomes and metabolomes of HD11.A blank control group(CONT group)and an APS treatment group(A50 group)were set up,in which HD11 cells of the A50 group was treated with complete culture medium contain 50μg·mL^(-1) APS for 12 h;and HD11 cells of the CONT group were cultured in complete culture medium for 12 h.Metabolomics and transcriptomic techniques were used to analyze the differential genes and differential metabolites in CONT and A50 treated cells,and the combined analysis of the two omics was performed.Results:A total of 845 differential genes were detected in the transcriptome analysis between the A50 treatment group and the CONT group,including 415 up-regulated genes and 430 down-regulated genes.Differentially expressed genes were mainly concentrated in cytokine-cytokine receptor interaction,toll-like receptor signaling pathway,phagosome and AGE-RAGE signaling pathways,among which cytokine-cytokine receptor interaction and toll-like receptor signaling pathways were the most significant.In the metabolome analysis and comparison between the A50 treatment group and the CONT group,a total of 89 differential metabolites were screened,of which 70 were down-regulated and 19 were up-regulated.In the combined analysis of transcription and metabolism of the A50 treatment group and the CONT group,the top ten trusted pathways were purine metabolism,carbon metabolism,pentose phosphate pathway,glutathione metabolism,histidine metabolism,amino sugar and nucleotide sugar metabolism,α-homolenic acid metabolism,ABC transporter,iron death and tyrosine metabolism.APS may exert immunomodulatory effects on HD11 cells through the metabolic reprogramming of toll-like receptor signaling pathway,glycolysis and pentose phosphate pathway.