基于生物信息学分析二仙汤治疗膝关节骨性关节炎合并骨质疏松症的机制

Mechanism of Erxian Decoction in the Treatment of Knee Osteoarthritis with Osteoporosis Based on Bioinformatics Analysis

目的通过生物信息学的方法探讨二仙汤对于骨质疏松症(OP)及膝关节骨性关节炎(KOA)共病治疗的可能机制,为临床应用提供理论依据。方法分别以骨质疏松症及膝关节骨性关节炎作为关键词,在Disgenet、TTD、Genecards疾病数据库筛选疾病靶点。在中药数据库平台筛选二仙汤的有效化合物,并通过Netinfer平台预测二仙汤的靶标,Venny2.1.0进行交集,获得二仙汤治疗OP合并KOA的靶点。通过String数据库制作靶点间相互作用PPI网络,并导入cytoscape3.7.2。使用R语言进行GO生物过程富集分析以及KEGG通路富集分析,并进行可视化。结果筛选OP相关靶点533个,KOA靶点302个,预测二仙汤靶点104个,取三者交集,获得二仙汤治疗OP合并KOA靶点共14个,GO生物学过程富集在炎症反应调节、胶原蛋白分解代谢过程等。KEGG富集在白介素17信号通道,雌激素信号通道等。结论二仙汤治疗OP合并KOA的主要机制可能是调节炎症因子、调节性激素水平,这为二仙汤进一步的临床应用及机制研究提供了依据。

Objective To explore the possible mechanism of Erxian decoction in the treatment of osteoporosis(OP)and knee osteoarthritis(KOA)by bioinformatics methods,and to provide theoretical basis for clinical application.Methods Osteoporosis and knee osteoarthritis were used as keywords to screen disease targets in Disgenet,TTD and Genecards disease databases.The effective compounds of Erxian decoction were screened on the traditional Chinese medicine database platform,and the target of Erxian decoction was predicted by Netinfer platform.Venny2.1.0 was used for intersection to obtain the target of Erxian decoction in the treatment of OP combined with KOA.The PPI network of interaction between targets was made through the String database and imported into cytoscape 3.7.2.GO biological process enrichment analysis and KEGG pathway enrichment analysis were performed using R language,and visualization was performed.Results A total of 533 OP-related targets,302 KOA targets,and 104 predicted Erxian decoction targets were screened.The intersection of the three was taken to obtain a total of 14 targets for the treatment of OP combined with KOA by Erxian decoction.GO biological processes were enriched in inflammatory response regulation,collagen catabolic process,etc.KEGG enrichment in interleukin 17 signal channel,estrogen signal channel,etc.Conclusion The main mechanism of Erxian decoction in the treatment of OP combined with KOA may be to regulate inflammatory factors and regulate sex hormone levels,which provides a basis for further clinical application and mechanism research of Erxian decoction..