急性硅肺小鼠模型系统评价

Systematic evaluation of acute silicosis mouse model

目的基于滴鼻法建立急性硅肺小鼠模型评价方法。方法小鼠麻醉后经鼻吸入的方法给予单次60μl二氧化硅50 mg/ml混悬液复制硅肺小鼠模型,以肺系数、肺干湿比、HE染色和免疫组织化学等作为硅肺小鼠肺损伤评价指标,Masson染色作为纤维化评价指标。结果与对照组相比,硅肺模型组小鼠在滴鼻1~5 d小鼠体重显著下降,运动能力有明显区分,且体重下降与运动距离存在显著相关性。硅肺模型组31 d,肺组织病理学观察到致密的斑片状病灶,提示肺部炎症脓肿并形成硅结节,伴有巨噬细胞和硅颗粒聚集;Masson染色显示纤维化显著,与二氧化硅吸入量呈剂量效应关系。结论采用单次经鼻吸入二氧化硅混悬液,30 d成功建立急性硅肺小鼠模型。

Objective To establish an evaluation method for acute silicosis mouse models based on intranasal administration.Methods Mice were given a single 60μl of silica suspension(50 mg/ml)through nasal inhalation after anesthesia to replicate the silicosis mouse model.Lung coefficient,lung dry/wet ratio,HE staining,immunohistochemistry were used as indicators for evaluating lung injury in silicosis mice.Masson staining was used as an indicator for fibrosis evaluation.Results Compared with the control group,mice in the model group showed significant weight loss and decreased motor ability from day 1~5 after intranasal administration.The weight loss was significantly correlated with distance traveled during movement.At day 31 in the model group,dense patchy lesions were observed in lung tissue pathology,indicating pulmonary inflammation abscesses and formation of silica nodules along with macrophage and silica particle aggregation.Masson staining revealed significant fibrosis which exhibited a dose-dependent relationship with SiO 2 inhalation dosage.Conclusions Acute silicosis mouse models were successfully established through intranasal administration within 30 days using this method.