宏基因组二代测序对肺部感染诊断价值的中国数据meta分析

Diagnostic value of metagenomics next-generation sequencing inpulmonary infection:A meta-analysis of Chinese data

目的系统评价宏基因组二代测序(mNGS)对肺部感染的诊断价值。方法检索PubMed、Embase、Web of Science、the Cochrane Library、中国知网、万方、维普等数据库,检索时限均为建库至2022年5月30日,按照纳入及排除标准筛选文献,对纳入文献进行质量评价,采用Meta-Disc1.4、RevMan5.3、STATA15.0软件进行统计学分析。结果共纳入12篇文献,共计1154例患者。Meta分析结果显示,合并敏感度为0.82[95%可信区间(95%CI)0.79~0.84,I^(2)=89.1%],合并特异度为0.73(95%CI 0.68~0.78,I^(2)=85.4%)。研究之间在敏感度和特异度方面的异质性较为显著。总受试者操作特征曲线(SROC)的曲线下面积(AUC)为0.850,Q指数为0.782。结论mNGS在肺部感染临床应用中的诊断性能尚可。

Objective To systematically evaluate the diagnostic value of metagenomic next-generation sequencing(mNGS)for detecting pulmonary infection.Methods PubMed,Embase,Web of Science,the Cochrane Library,CNKI,WanFang,VIP databases were searched for studies according to the criteria of inclusion and exclusion,enrolling literature recorded from database establishment to May 30,2022.Statistical analysis was performed by Meta-Disc1.4,RevMan5.3 and STATA15.0 software.Results A total of 12 relevant studies were enrolled,including 1154 patients.The results of meta-analysis showed that the pooled sensitivity was 0.82(95%CI 0.79-0.84,I^(2)=89.1%),and the pooled specificity was 0.73(95%CI 0.68-0.78,I^(2)=85.4%).The heterogeneity in sensitivity and specificity between studies was significant.The area under the curve(AUC)of the total subject operating characteristic curve(SROC)was 0.850,and the Q index was 0.782.Conclusion The diagnostic performance of mNGS in the clinical application of lung infections is acceptable.