长链非编码RNA H19、CHAST在心房颤动患者血清中的表达水平及临床意义

Expression and clinical significance of serum long non-coding RNA H19 and CHAST in patients with atrial fibrillation

目的 探讨心房颤动患者血清长链非编码RNA(lncRNA)H19、lncRNA CHAST的表达水平及临床意义。方法 选取心房颤动患者100例(阵发性心房颤动50例,非阵发性心房颤动50例)作为研究组,同期50例健康体检成年人作为对照组。收集所有受试者的一般临床资料及相关检查指标,采用实时荧光定量PCR法检测血清lncRNA H19、lncRNA CHAST的表达水平,评估心房颤动患者血清lncRNA H19、lncRNA CHAST表达水平与心肌重构相关指标的相关性;Logistic回归分析确定心房颤动的影响因素;分析血清lncRNA H19、lncRNA CHAST表达水平对心房颤动的预测价值。结果 研究组血清lncRNA H19和lncRNA CHAST表达水平高于对照组(1.64±0.48和1.58±0.40 vs.1.05±0.33和1.06±0.35,P<0.05)。非阵发性心房颤动组血清lncRNA H19和lncRNA CHAST表达水平高于阵发性心房颤动组(P<0.05)。lncRNA H19、lncRNA CHAST表达水平与左心房内径、左心室舒张末内径呈正相关,和左心室射血分数呈负相关(P<0.05)。Logistic回归分析结果显示,lncRNA H19表达水平(OR=5.115,95%CI:1.240~21.089)和lncRNA CHAST表达水平(OR=2.522,95%CI:1.172~5.426)是心房颤动的影响因素。ROC曲线显示,lncRNA H19、lncRNA CHAST的表达水平及其联合预测心房颤动的曲线下面积分别是0.860、0.833、0.932。结论 lncRNA H19、lncRNA CHAST为心房颤动的独立危险因素,两指标对预测心房颤动发生有一定的价值,且两者联合对预测心房颤动发生的价值更高。

Objective To investigate the expression levels and clinical significance of serum long non-coding RNA(lncRNA) H19 and lncRNA CHAST in patients with atrial fibrillation.Methods In total,100 patients with atrial fibrillation(50 patients with paroxysmal atrial fibrillation and 50 patients with non-paroxysmal atrial fibrillation) were selected as the research group,and 50 healthy adults who underwent physical examination during the same period were selected as the control group.General clinical data and relevant examination indicators from all subjects were collected,and real-time fluorescence quantitative PCR(RT-qPCR) method was used to detect the expression levels of serum lncRNA H19 and lncRNA CHAST,and the correlation between the expression levels of serum lncRNA H19 and lncRNA CHAST and myocardial remodeling-related indicators in patients with atrial fibrillation was evaluated.Logistic regression analysis was used to determine the influencing factors of atrial fibrillation,and the predictive value of serum lncRNA H19 and lncRNA CHAST expression levels for atrial fibrillation was analyzed.Results The serum levels of lncRNA H19 and lncRNA CHAST expression in the research group were higher than those in the control group(1.64±0.48 and 1.58±0.40 vs.1.05±0.33 and 1.06±0.35,P<0.05).The serum levels of lncRNA H19 and lncRNA CHAST expression in the non-paroxysmal atrial fibrillation group were higher than those in the paroxysmal atrial fibrillation group(P<0.05).The expression levels of lncRNA H19 and lncRNA CHAST were positively correlated with left atrial diameter and left ventricular end-diastolic diameter,and negatively correlated with left ventricular ejection fraction(P<0.05).The results of logistic regression analysis showed that the expression levels of lncRNA H19(OR=5.115,95%CI:1.240-21.089) and lncRNA CHAST(OR=2.522,95%CI:1.172-5.426) were influencing factors for atrial fibrillation.The ROC curve showed that the AUC of expression levels of lncRNA H19 and lncRNA CHAST alone and in combination for predicting atrial fibrillation were 0.860,0.833,and 0.932,respectively.Conclusion lncRNA H19 and lncRNA CHAST are independent risk factors for atrial fibrillation.The two indicators have certain value in predicting the occurrence of atrial fibrillation,and the combination of the two has higher value in predicting the occurrence of atrial fibrillation.