血清IL-33和TNF-α活性及外周血淋巴细胞中NF-κB水平与冠心病不同程度病变的相关性分析

Correlation analysis of serum IL-33,TNF-αactivity and NF-kB level in peripheral blood lymphocytes with different degrees of coronary heart disease

目的:分析冠心病(CHD)冠状动脉病变严重程度与血清白细胞介素33(IL-33)和肿瘤坏死因子α(TNF-α)活性水平及外周血淋巴细胞中核因子κB(NF-κB)蛋白表达之间的相关性,探讨其在CHD发展中的作用及价值。方法:选择就诊于心内科并行冠状动脉造影检查并确诊为CHD的病人167例(CHD组),同时期行冠脉造影正常对照27例(CON组)。根据Gensini评分将167例CHD病人分成轻微病变组(<30分,LS组)84例、中度病变组(30~<60分,MS组)54例和重度病变组(≥60分,HS组)29例。实验室行ELISA检测血清中IL-33和TNF-α活性水平,分析在不同冠状动脉病变程度下IL-33和TNF-α的活性水平差异,探讨IL-33和TNF-α活性水平与CHD的关联性。同时取CHD组及CON组的外周血淋巴细胞,提取蛋白,使用Western blotting检测NF-κB蛋白表达情况。结果:HS组血清总胆固醇(TC)与CON组和LS组、MS组相比升高(P<0.05),HS组血清IL-33与TNF-α水平较MS组、LS组和对照组升高,并且随着冠状动脉病变程度的加重逐渐升高(P<0.01),两者之间呈正相关关系(r=0.663,P<0.01);CHD组中NF-κB蛋白表达低于CON组(P<0.01)。结论:随着冠状动脉病变程度的加重,血清IL-3和TNF-α水平也逐渐升高,且两者间存在一定程度的线性相关,同时与CON组相比,CHD病人NF-κB蛋白表达显著升高。

Objective:To analyze the correlation of serum interleukin 33(IL-33),tumor necrosis factor α(TNF-α)levels and nuclear factorκB(NF-κB)protein expression in peripheral blood lymphocytes with the severity of coronary heart disease(CHD),and explore its role and value in the development of CHD.Methods:A total of 167 patients(CHD group)admitted to Department of Cardiology with coronary angiography and confirmed as CHD were selected,and during the same period,27 cases with normal coronary angiography were confirmed as control group(CON group).According to Gensini score,167 CHD patients were divided into mild lesion group(<30 points,LS group,84 cases),moderate lesion group(≥30 points to<60 points,MS group,54 cases),and severe lesion group(≥60 points,HS group,29 cases).Serum IL-33 and TNF-α levels were detected by ELISA,the differences of IL-33 and TNF-αlevels in different degrees of coronary artery disease were analyzed,and the correlation between IL-33,TNF-α levels and CHD was analyzed.Meanwhile,the peripheral blood lymphocytes in CHD group and CON group were collected for protein extraction,and NF-κB protein expression was detected by Western blotting.Results:Serum total cholesterol(TC)level in HS group was increased compared with that in CON group,LS group and MS group(P<0.05),serum IL-33 and TNF-α levels in HS group were increased compared with those in MS group,LS group and CON group,and these parameters were gradually increased with the aggravation of coronary artery disease(P<0.01).There was a positive correlation between serum IL-33,TNF-α levels and coronary artery disease(r=0.663,P<0.01).The NF-κB protein expression in CHD group was significantly higher than that in CON group(P<0.01).Conclusions:With the increasing degree of coronary artery disease,serum IL-3 and TNF-α levels were gradually increased,and there was a certain degree of linear correlation.NF-κB protein expression in CHD group was significantly increased compared with that in CON group.