miR-595、miR-1246在溃疡性结肠炎血清中的表达及其与肠道菌群相关性研究

Expression of miR-595 and miR-1246 in serum of ulcerative colitis and its correlation with intestinal flora

目的:探讨miR-595、miR-1246在溃疡性结肠炎血清中的表达及其与肠道菌群相关性。方法:选取溃疡性结肠炎病人160例作为观察组,并根据疾病活动性分为活动期(aUC组)和缓解期(rUC组),各80例。同期选取健康体检者158名作为对照组。采用实时荧光定量PCR法检测各组血清中miR-595、miR-1246表达水平;采用ELISA法检测各组血清肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6、IL-22水平;采集各组新鲜粪便,使用梯度稀释法对菌群进行培养。结果:aUC组和rUC组病人miR-595、miR-1246表达和TNF-α、IL-6、IL-22水平均高于对照组(P<0.05),aUC组亦均高于rUC组(P<0.05)。aUC组和rUC组乳酸杆菌、双歧杆菌数量均低于对照组(P<0.05),aUC组亦均低于rUC组(P<0.05);aUC组肠球菌、大肠埃希菌数量均明显高于对照组和rUC组(P<0.05)。Pearson相关分析显示,miR-595、miR-1246表达水平和TNF-α、IL-6、IL-22水平与乳酸杆菌、双歧杆菌数目均呈负相关关系(P<0.05),而以上指标与肠球菌、大肠埃希菌数目均呈正相关关系(P<0.05)。结论:溃疡性结肠炎病人血清中miR-595、miR-1246表达上调,且与肠道菌群数量存在相关关系。

Objective:To explore the expression of miR-595 and miR-1246 in serum of ulcerative colitis and its correlation with intestinal flora.Methods:A total of 160 patients with ulcerative colitis were selected as the observation group.According to the disease activity,the patients were divided into active phase(aUC group)and remission phase(rUC group),with 80 cases in each group.During the same period,158 healthy people for physical examination were selected as the control group.Real-time fluorescent quantitative PCR(RT-qPCR)was used to detect the expression levels of serum miR-595 and miR-1246.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and interleukin-22(IL-22)levels.Fresh feces of patients and healthy people were collected,and gradient dilution method was used to culture and analyze the flora.Results:The expression levels of miR-595,miR-1246,TNF-α,IL-6 and IL-22 in the aUC and rUC group were significantly higher than those in the control group(P<0.05),and which in the aUC group were higher than those in the rUC group(P<0.05).The number of lactobacillus and bifidobacteria in the aUC group and rUC group were lower than those in the control group(P<0.05),and which in the aUC group were lower than those in the rUC group(P<0.05).The number of enterococcus and Escherichia coli in aUC group was significantly higher than that in control group and rUC group(P<0.05).Pearson correlation analysis showed that the expression levels of miR-595,miR-1246,TNF-α,IL-6 and IL-22 were negatively correlated with the number of lactobacilli and bifidobacteria(P<0.05),and positively correlated with the number of enterococci and Escherichia coli(P<0.05).Conclusions:The expression of miR-595 and miR-1246 in the serum of ulcerative colitis is significantly up-regulated,and closely related to the number intestinal flora.