高通量测序技术在肺真菌病诊断中的研究进展

Research progress of high-throughput sequencing in the diagnosis of pulmonary fungal diseases

肺真菌病是一种病死率较高的肺部炎症性疾病,其临床表现缺乏特异性,早期诊断困难。传统的真菌检测方法的检出率和诊断准确性受到一定限制。高通量测序又称为下一代测序技术或二代测序技术,是一种新型的病原体检测手段,可以同时检测多个微生物的核酸序列,从而具有在混合感染和罕见真菌感染的诊断中更有优势的特点。高通量测序技术仍存在局限性,需要结合临床区分定植菌和致病菌。

Pulmonary fungal disease is a kind of pulmonary inflammatory disease with high fatality.The lack of specificity in clinical manifestations leads to difficult early diagnosis.The detection rate and diagnostic accuracy of traditional fungal detection methods are limited.High-throughput sequencing,also known as next-generation sequencing(NGS),is a new method for pathogen detection,which can detect the nucleic acid sequences of multiple microorganisms at the same time.Therefore,it has more advantages in the diagnosis of mixed infection and rare fungal infections.NGS technology still has some limitations,however,and it is necessary to distinguish colonized fungi from pathogenic fungi based on clinical practice.