百香果提取液绿色制备银纳米粒子及其抗菌研究

Green preparation of silver nanoparticles from passion fruit extract and their antibacterial research

目前,人们已经提出了很多用于合成银纳米粒子的化学方法,其中绿色合成银纳米粒子的方法由于其低成本和环境友好性而受到更多关注。该研究报道了以百香果果皮提取物为原料绿色合成银纳米粒子的方法并研究其抗菌性能。通过紫外可见光谱在420 nm处出现峰值来确定银纳米颗粒的形成,以透射电子显微镜和扫描电子显微镜表征其粒径大小及表面形态,纳米银(AgNPs)多呈球形,分散性良好,平均粒径为12.1 nm;采用X射线衍射分析AgNPs结晶性质并证明了AgNPs的存在;使用光电子能谱仪分析Ag价态为0价;热重分析仪测定结果表明了AgNPs有良好的热稳定性;傅里叶红外光谱及拉曼散射光谱测试结果显示,百香果果皮提取物具有还原和稳定银纳米粒子的双重功能,其中具有该作用的活性物质可能是蛋白质、多酚、黄酮和果胶等。大肠杆菌和金黄色葡萄球菌最小抑制质量浓度分别为33、66μg/mL。综上所述,利用百香果提取液可以绿色制备较为理想的AgNPs,有望在制药、工业等抑菌领域得到广泛应用。

In this study,silver nanoparticles(AgNPs)were synthesized from passion fruit peel extract and their antimicrobial properties were studied.The formation of AgNPs was determined by a UV-Vis peak at 420 nm.Transmission electron microscope(TEM)and scanning electron microscope(SEM)were used to characterize the particle size and surface morphology of AgNPs.Most of the AgNPs were spherical with good dispersion and the average particle size was 12.1 nm.X-ray diffraction(XRD)analysis of AgNPs analyzed the crystallization properties of AgNPs and demonstrated the presence of AgNPs.The thermogravimetric analyser(TGA)indicated good thermal stability of the AgNPs.Fourier transform infrared spectroscopy(FTIR)and Raman scattering spectroscopy results showed that the passion fruit peel extract had the dual functions of reducing and stabilizing silver nanoparticles,among which the active substances might be protein,polyphenols,flavonoids,and pectin.The minimum inhibitory concentration of Escherichia coli and Staphylococcus aureus were 33.3μg/mL and 66.7μg/mL,respectively.In conclusion,the ideal nano-silver can be prepared green by using passion fruit extract,which is expected to be widely used in antibacterial fields such as pharmacy and industry.