草麻黄根中一个新软木脂类化合物

A new suberin from roots of Ephedra sinica Stapf

采用硅胶柱色谱、薄层色谱和半制备液相等多种色谱学技术从草麻黄根中分离得到6个化合物。根据理化性质与波谱数据鉴定其结构,分别为(Z)-阿魏酸二十二酯(1)、(E)-阿魏酸二十二酯(2)、邻苯二甲酸-双(2′-乙基庚基)酯(3)、2,2′-氧代双(1,4-二叔丁苯)(4)、邻苯二甲酸二异丁酯(5)、邻苯二甲酸二(2-乙基己基)酯(6)。其中化合物1为新化合物,化合物2~4是首次从麻黄植物中分离得到。采用皮质酮诱导大鼠肾上腺嗜铬细胞瘤(PC-12细胞)细胞损伤模型进行化合物活性筛选,结果表明,化合物1和5能显著改善皮质酮诱导的PC-12细胞损伤,并且显著升高细胞中5-HT7(5-hydroxytryptamine 7)受体蛋白的表达,具有潜在的抗抑郁活性。

Six compounds were isolated from the roots of Ephedra sinica Stapf using various chromatographic techniques such as silica gel column chromatography,thin layer chromatography and semi-preparative HPLC.Their chemical structures were identified by analysis of physicochemical properties and spectral data,and determined as(Z)-docosanylferulate(1),(E)-docosanylferulate(2),bis(2-ethylheptyl)phythalate(3),2,2′-oxybis(1,4-di-tertbutylbenzene)(4),diisobutyl phthalate(5),bis(2-ethylhexyl)phthalate(6).Among them,compound 1 is a new compound,compounds 2−4 were first isolated from Ephedra.A corticosterone-induced PC-12 cell injury model was used for compound activity screening.The results showed that compounds 1 and 5 significantly improved corticosterone-induced PC-12 cell injury and significantly increased 5-HT7 receptor protein expression in the cells,indicating potential antidepressant activity.