酶底物光谱法与酶底物法检测水中大肠菌群的比较分析

Comparative Analysis of Enzyme Substrate Spectrometry and Enzyme Substrate Method for Determination of Coliform Bacteria in Water

采用新型酶底物光谱法和酶底物法对不同类型水样中总大肠菌群、大肠埃希氏菌及粪大肠菌群指标进行同步检测。两种方法各有优势,采用SPSS软件对检测结果处理后进行配对t检验,结果显示:两组总大肠菌群的数据P=0.057,相关系数r=0.904;两组大肠埃希氏菌的数据P=0.593,相关系数r=0.972;两组粪大肠菌群的数据P=0.136,相关系数r=0.986,表明两种检测方法具有相关性,且没有统计学意义上的显著性差异。采用酶底物光谱法检测有证质控样品,总大肠菌群、大肠埃希氏菌、粪大肠菌群的检测结果均在质控样真值范围内,证明该方法检测准确度符合要求。酶底物光谱法与酶底物法相比,反应原理类似,操作更加简便,具有仪器自动化和智能化快速检测、判读、存储、联网传输数据等优点,可满足实验室常规检测需求,在应急突发事件现场检测方面有较强的运用前景和优势。

Simultaneous determination of total coliforms,Escherichia coli,and fecal coliforms in different types of water samples was carried out using novel enzyme substrate spectrometry and enzyme substrate method.Both methods have their own advantages.After processing the detection results using SPSS software,paired t-tests were conducted.The results showed that the P value of the two groups of determination of total coliforms was 0.057,and the correlation coefficient was 0.904.The P value of two groups of Escherichia coli was 0.593,and the correlation coefficient was 0.972.The P value of the two groups of fecal coliforms was 0.136,and the correlation coefficient was 0.986.It indicated a correlation between the two detection methods,and there was no statistically significant difference.The enzyme substrate spectrometry method was used for detecting certified quality control samples,and the detection results of determination of total coliforms,Escherichia coli,and fecal coliforms were all within the true value range of the quality control sample,proving that the detection accuracy of this method met the requirements.Compared with enzyme substrate method,enzyme substrate spectrometry has similar reaction principles and is more convenient to operate.It has advantages such as instrument automation and intelligent rapid detection,interpretation,storage,and online transmission of data.It can meet the needs of routine laboratory testing and has strong application prospects and advantages in emergency on-site testing.