经后增殖方对控制性超促排卵大鼠卵巢GDF9分泌及颗粒细胞凋亡的影响

Effect of Jinghou Zengzhi Granules on ovarian GDF9 secretion and granulosa cells apoptosis in controlled ovarian hyperstimulation rats

目的观察益气血法经后增殖方通过p38MAPK/CK2/IκBα/NF-κB通路对控制性超促排卵(COH)大鼠卵巢GDF9分泌及颗粒细胞(GCs)凋亡的影响。方法建立COH大鼠模型,18只大鼠随机分为自然排卵组(NO组)、COH组、COH+经后增殖方组(COH+JHZZG组)。qRT-PCR和Western blot法检测p38MAPK、CK2、IκBα、NF-κB、GDF9 mRNA和蛋白的表达水平,TUNEL法检测卵巢GCs凋亡率。结果与NO组比较,COH组大鼠卵巢组织p38MAPK、NF-κB表达升高,CK2、IκBα、GDF9表达下降,卵巢GCs凋亡率升高,差异有统计学意义(P<0.01);与COH组比较,COH+JHZZG组大鼠卵巢组织p38MAPK、NF-κB表达下降,CK2、IκBα、GDF9表达升高,差异有统计学意义(P<0.01);卵巢GCs凋亡率下降,差异有统计学意义(P<0.05)。结论益气血法经后增殖方通过p38MAPK/CK2/IκBα/NF-κB通路促进COH大鼠卵巢GDF9的分泌,抑制卵巢GCs的凋亡,从而提高COH卵细胞质量。

Objective To explore the effects of Jinghou Zengzhi Granules(JHZZG),on ovarian GDF9 secretion and granulosa cells(GCs)apoptosis in controlled ovarian hyperstimulation(COH)rats through p38MAPK/CK2/IκBα/NF-κB pathway.Methods A model of COH rats was prepared and 18 rats were randomly divided into 3 groups,including natural ovulation group(NO group),COH group,and COH+JHZZG group.The expression of p38MAPK,CK2,IκBα,NF-κB,GDF9 mRNA and protein were detected by qRT-PCR and Western blot,and the apoptosis rate of ovarian GCs by TUNEL.Results Compared with the NO group,the expression of p38MAPK and NF-κB in the ovarian tissue of rats in the COH group increased,while the expression of CK2,IκBαand GDF9 decreased,and the apoptosis rate of ovarian GCs increased(all P<0.01).Compared with the COH group,the expression of p38MAPK and NF-κB in the ovarian tissue of rats in the COH+JHZZG group decreased,while the expression of CK2,IκBαand GDF9 increased(all P<0.01),and the apoptosis rate of ovarian GCs decreased(P<0.05).Conclusion Jinghou Zengzhi Granules could promote the secretion of ovarian GDF9 and inhibit the apoptosis of ovarian GCs of COH rats through p38MAPK/CK2/IκBα/NF-κB signaling pathway,which thereby improve the quality of COH oocytes.