广陈皮精油的抗氧化活性研究及其应用

Study on Antioxidant Activity of Pericarpium Citrus Reticulata Essential Oil and Its Application

本文以超临界CO_(2)萃取法提取的5年份广陈皮精油为研究对象,利用气相色谱-质谱联用技术对广陈皮精油化学组成成分进行分析,采用体外抗氧化测定方法来综合评价广陈皮精油的抗氧化能力,以壳聚糖包埋方法制备了一种新型的精油纳米乳液并进行应用评价。结果表明:广陈皮精油中有20种主要挥发性成分,其中柠檬烯含量最高(58.41%)。体外抗氧化活性测定结果显示广陈皮精油对ABTS自由基(IC_(50)=5.75 mg/mL)和DPPH自由基(IC_(50)=44.54 mg/mL)均具有一定的清除能力,具有良好的抗氧化活性。扫描电镜(SEM)形态观察结果表明广陈皮精油纳米乳液呈球形,均匀度稳定性良好。在实际应用中,精油纳米乳液可有效延缓苹果的氧化褐变,达到防腐保鲜的效果。

In this study,the essential oil(EO)of Pericarpium Citri Reticulatae(PCR,aged for 5 years)was extracted by supercritical CO_(2)extraction.The components of EO were analyzed by gas chromatography-mass spectrometry(GC-MS),and the antioxidant capacity of essential oil was evaluated by in vitro antioxidant assay.A new kind of nanoemulsion for essential oil was prepared by chitosan embedding method.The results showed that about 20 volatile components identified in the essential oil of PCR,and the main component was limonene(58.41%).The results of antioxidant activity in vitro showed that essential oil of PCR had good antioxidant ability,which had scavenging abilities on ABTS free radical(IC_(50)=5.75 mg/mL)and DPPH free radical(IC_(50)=44.54 mg/mL),respectively.The scanning electron microscope(SEM)result showed that the nanoemulsion was spherical,and had good uniformity and stability.In practical application,the essential oil nanoemulsion can effectively delay the oxidation Browning of apple and achieve the effect of preservation.The results indicated that the emulsion had broad prospects in the development and utilization of natural plant food preservatives.