金霉素与土霉素对生物除磷中微生物的影响

Effects of Chlortetracycline and Oxytetracycline on Microorganisms in Biological Phosphorus Removal

为揭示金霉素和土霉素混合物对生物除磷系统中微生物的影响,文章采用直接均分射线法设计配比为R1、R2和R3的金霉素和土霉素的混合物。通过高通量测序技术分析不同配比的混合物对微生物的影响,运用RStudio技术分析环境因子与微生物间的相关性,借助R-4.1.0软件和Gephi可视化分析微生物间相互关系。结果表明:R1配比下的微生物对环境因子的反应更为敏感,显著性更强;R2配比下的微生物结构更稳定,菌属之间的联系更为密切;3种配比情况下生物除磷菌丰度均下降。其中,Acinetobacter菌属丰度在R3时减少最明显,减少了36.11%。研究表明,不同浓度配比的金霉素与土霉素对生物除磷系统中微生物的影响有较大差异,主要因为不同占比下金霉素与土霉素影响微生物群落结构,进而影响生物除磷效果。

To reveal the effect of chlortetracycline and oxytetracycline on microorganisms in biological phosphorus removal system,three mixtures of chlortetracycline and oxytetracycline with different ratios(R1,R2 and R3)were designed by direct equipartition radiography.The effects of three mixtures on microorganisms were analyzed by high-throughput sequencing technology,the correlation between environmental factors and microorganisms was analyzed by RStudio,and the interrelation⁃ship between microorganisms was visualized with R-4.1.0 software and Gephi.The results show that when the ratio is R1,microorganisms are more sensitive and significant in response to environmental factors.When the ratio is R2,the correlation between microbial structure is more stable,and the correlation between microorganisms is closer.The abundance of biological phosphorus removal bacteria decreased under the three ratios.Among them,the abundance of Acinetobacter decreased most significantly at R3,with a decrease of 36.11%.Research showed that there were significant differences in the effects of differ⁃ent ratios of chlortetracycline and oxytetracycline on microorganisms during biological phosphorus removal systems.