百合鸡子汤对抑郁症大鼠垂体代谢物谱的影响研究

Effect of Baihe Jizi decoction on the pituitary metabolite profile of rats with depression

目的:运用核磁共振代谢组学技术分析慢性不可预知温和刺激(CUMS)抑郁模型大鼠垂体组织中小分子化合物的代谢变化,探讨百合鸡子汤可能影响的相关代谢途径。方法:采用CUMS建立抑郁模型,取大鼠垂体组织进行核磁共振检测,采用代谢组学多元统计分析方法对相关差异代谢物进行可视化分析,并通过差异代谢物的含量变化来确定百合鸡子汤可能影响的代谢途径。结果:主成分分析表明组间差异明显,结合多元统计分析,筛选出乳酸、谷氨酰胺、胆碱等8种差异代谢物。相较于正常组,乳酸、丙氨酸、谷氨酸等代谢物的含量在模型组垂体中呈下降趋势(P<0.05或P<0.01);相较于模型组,乳酸、丙氨酸、谷氨酸等代谢物的含量在百合鸡子汤中剂量组垂体中呈上升趋势(P<0.05或P<0.01)。结论:抑郁能够显著改变大鼠垂体组织的代谢物谱,百合鸡子汤可能通过丙氨酸、天冬氨酸、谷氨酸代谢,D-谷氨酸、D-谷氨酰胺代谢,精氨酸合成,牛磺酸、亚牛磺酸代谢等途径发挥抗抑郁作用。

Objective:To investigate the changes in the metabolism of small-molecule compounds in pituitary tissue of a rat model of depression induced by chronic unpredicted mild stress(CUMS),as well as the metabolic pathways affected by Baihe Jizi decoction.Methods:CUMS was used to establish a model of depression.The pituitary tissue of rats was collected for magnetic resonance;the metabolomics multivariate statistical analysis was used to perform visual analysis of differentially expressed metabolites,and changes in the content of differentially expressed metabolites were used to determine the metabolic pathways affected by Baihe Jizi decoction.Results:The principal component analysis showed significant differences between groups,and eight differentially expressed metabolites,including lactic acid,glutamine,and choline,were obtained by the multivariate statistical analysis.Compared with the normal group,the model group had significant reductions in the content of the metabolites such as lactic acid,glutamine,and choline(P<0.05 or P<0.01),and compared with the model group,the middle-dose Baihe Jizi decoction group had significant increases in the content of these metabolites(P<0.05 or P<0.01).Conclusion:Depression can significantly change the metabolite profile of pituitary tissue in rats,and Baihe Jizi decoction can exert an antidepressive effect through the pathways such as alanine,aspartate,and glutamate metabolism,D-glutamine and D-glutamic acid metabolism,and taurine and hypotaurine metabolism.