日粮中添加赶黄草提取物对免疫应激肉仔鸡炎症反应的影响及其肝脏保护作用

Effect of dietary supplementation of Penthorum chinense Pursh extract on inflammatory response and hepatoprotective effect in immune-stressed broilers

为了探究赶黄草提取物对肉仔鸡免疫应激的影响,将60只1日龄红羽肉鸡分为对照(Control)组、脂多糖(lipopolysaccharides,LPS)组和赶黄草提取物(Penthorum chinense Pursh,PCP)治疗组。LPS组和PCP组分别在12、14、33和35日龄腹腔注射250μg/kg的LPS诱导免疫应激,对照组注射等量的无菌生理盐水。PCP组在基础日粮中添加5 g/kg赶黄草提取物,试验周期为35 d。在第2、5周,每组随机选择5只鸡,经颈静脉采血,在最后1次LPS注射12 h后,每组随机选择10只鸡,以颈椎脱臼的方式处死,收集肝脏。结果显示,PCP显著降低了血清中促肾上腺皮质激素(ACTH)和皮质酮(CORT)的水平,提高了生长激素(GH)的水平;降低了血清中γ干扰素(IFN-γ)、诱导型一氧化氮合酶(iNOS)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及肿瘤坏死因子-β(TNF-β)的含量,提高了血清中白细胞介素-10(IL-10)的含量;有效缓解了LPS引起的肝脏损伤,降低了血清和肝脏中丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)的水平;提高了肝脏中谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)、过氧化氢酶(CAT)及总超氧化物歧化酶(T-SOD)的含量。结果表明,基础日粮中添加PCP有效缓解了LPS诱导肝脏损伤和炎症反应,缓解了LPS诱导的免疫应激,该研究为肉鸡免疫应激提供有效的防治方案,并为PCP在肉鸡炎症相关性疾病中的应用提供依据。

To investigate the effect of the LPS extract on immune stress in broilers,60 red-finned broilers were divided into a control(Control)group,a lipopolysaccharides(LPS)group and a Penthorum chinense Pursh(PCP)group.The PCP group was treated with 5 g/kg of PCP extract in the basal diet for 35 d.At weeks 2 and 5,5 chickens in each group were randomly selected and blood was collected via the jugular vein.12 h after the last LPS injection,10 chickens in each group were randomly selected and executed by cervical dislocation and the livers were collected.The results showed that PCP significantly reduced serum levels of adrenocorticotropic hormone(ACTH)and corticosterone(CORT)and increased levels of growth hormone(GH);reduced serum levels of gamma interferon(IFN-γ),inducible nitric oxide synthase(iNOS),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and tumor necrosis factor-β(TNF-β)and increased serum levels of interleukin-10(IL-10);effectively alleviated LPS-induced liver injury and reduced serum and liver levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST);increased liver levels of glutathione peroxidase(GSH-Px),total antioxidant capacity(T-AOC),catalase(CAT)and total superoxide dismutase(T-SOD)levels in the liver.In conclusion,the addition of PCP to the basal diet effectively alleviated LPS-induced liver damage and inflammatory response and alleviated LPS-induced immune stress.The study provides an effective control protocol for immune stress in broilers and provides a basis for the application of PCP in broiler inflammation-related diseases.