白花蛇舌草提取物对肝癌术后感染大鼠PI3K/AKT/mTOR信号通路的调控作用

Effect of Hydnoglossus alba extract on regulation of PI3K/AKT/mTOR signaling pathways of hepatocellular carcinoma rats with postoperative infection

目的 分析白花蛇舌草提取物对肝癌术后感染大鼠磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(AKT)/哺乳动物雷帕霉素靶蛋白(m TOR)信号通路的调控作用。方法 选取SPF级Wistar大鼠44只,10只为空白组,剩余34只建立肝癌术后感染模型,共有30只大鼠建模成功,将其分为模型组、药物对照组、白花蛇舌草提取物组3组,每组10只。药物对照组给予阿莫西林克拉维酸钾,白花蛇舌草提取物组给予白花蛇舌草提取物,空白组、模型组不做处理,干预7d后观察大鼠变化。结果 与空白组相比,模型组、药物对照组、白花蛇舌草提取物组外周血C-反应蛋白(CRP)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、丙氨酸氨基转移酶(ALT)、总胆红素(TBil)、天冬氨酸氨基转移酶(AST)、总胆汁酸(TBA)水平、PI3K、AKT、mTOR mRNA表达量及相对表达量上升(P<0.05);与模型组相比,药物对照组、白花蛇舌草提取物组CRP、IL-8、TNF-α、ALT、TBil、AST、TBA水平、PI3K、AKT、mTOR mRNA表达量及相对表达量下降(P <0.05);与药物对照组相比,白花蛇舌草提取物组CRP、IL-8、TNF-α、ALT、TBil、AST、TBA水平、PI3K、AKT、mTOR mRNA表达量及相对表达量下降(P <0.05)。结论 肝癌术后感染大鼠经白花蛇舌草提取物干预后炎症反应减轻,肝损伤缓解,其机制可能与PI3K/AKT/mTOR信号通路受抑有关。

OBJECTIVE To observe the effect of Hydnoglossus alba extract on regulation of phosphatidylinositol 3 kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR)signaling pathways of hepato-cellular carcinoma rats with postoperative infection.METHODS Totally 44 Wistar rats of SPF grade were enrolled in the study,10 of which were assigned as the bland group,the rest of 34 were chosen to build up the models of postoperative infection.A total of 30 rats were successfully built up for the models and were divided into the model group,the drug control group and the Hedyotis alba extract group,with 10 rats in each group.The drug control group was treated with amoxicillin and clavulanate potassium,the Hedyotis alba extract group was given Hydno-glossum extract;the blank group and the model group were not given any treatment.The changes of the rats were observed after the interventions were taken for 7 days.RESULTS The levels of C-reactive protein(CRP),interleu-kin-8(IL-8),tumor necrosis factor-α(TNF-α),alanine aminotransferase(ALT),total bilirubin(TBiL)and total bile acid(TBA)as well as the mRNA expression levels and relative expression levels of PI3K,AKT and mTOR were significantly higher in the model group,the drug control group and the Hedyotis alba extract group than in the blank group.(P<0.05).The levels of CRP,IL-8,TNF-α,ALT,TBil,AST and TBA as well as the mRNA expression levels and relative expression levels of PI3K,AKT and mTOR were significantly lower in the drug con-trol group and the Hedyotis alba extract group than in the model group(P<0.05).The levels of CRP,IL-8,TNF-α,ALT,TBil,AST and TBA as well as the mRNA expression levels and relative expression levels of PI3K,AKT and mTOR were significantly lower in the Hedyotis alba extract group than in the drug control group(P<0.05).CONCLUSION Hydnoglossus alba extract may alleviate the inflammatory reactions of the hepatocellular carcinoma rats with postoperative infection and relieve the liver damage,and the mechanisms may be associated with the inhibition of PI3K/AKT/mTOR signaling pathways.